概述

  • 产品名称Anti-TFIIB抗体[IIB8]
    参阅全部 TFIIB 一抗
  • 描述
    小鼠单克隆抗体[IIB8] to TFIIB
  • 经测试应用适用于: Functional Studies, EMSA, ELISA, IP, Purification, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Partially purified recombinant human transcription factor IIB (TFIIB, Promega Corp.).

  • 表位Amino acids 52-105 of human and mouse TFIIB.

性能

应用

Our Abpromise guarantee covers the use of ab819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution. This antibody can also immuno-deplete TFIIB from cell nuclear extracts and inhibit transcription of TFIIB from cell nuclear extracts.
EMSA Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
Purification Use at an assay dependent dilution. This antibody is polyol-responsive and can be used for gentle purification of TFIIB.
WB Use at an assay dependent dilution. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).

靶标

Anti-TFIIB antibody [IIB8] (ab819)参考文献

This product has been referenced in:
  • Paes de Faria J & Fernandes L Protection against oxidative stress through SUA7/TFIIB regulation in Saccharomyces cerevisiae. Free Radic Biol Med 41:1684-93 (2006). WB ; Saccharomyces cerevisiae . Read more (PubMed: 17145557) »
  • Thompson NE  et al. Accessibility of epitopes on human transcription factor IIB in the native protein and in a complex with DNA. J Biol Chem 270:4735-40 (1995). Read more (PubMed: 7533165) »

See all 3 Publications for this product

Product Wall

Thank you for getting back to us and sharing your results. We have looked at our database but did not receive any similar complaint so far. We are very sorry to inform you that we have sold out all the vials from Lot: 12091. However, we have new ba...

Read More

Thank you for your message. We have had no other complaints regarding this antibody or about other batches. I'm happy that you have had success with ab819 in the past and would like to reassure you that our policy is that if you happened to have proble...

Read More

Thank you for your message. We encourage customers to feed their results back to us, whether positive or negative, and make all information about each of our products available to researchers. Please submit your review, in return we will forward a...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"