概述

  • 产品名称Anti-TFIIB抗体
    参阅全部 TFIIB 一抗
  • 描述
    兔多克隆抗体to TFIIB
  • 特异性This antibody recognizes TFIIB
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: SSDRAMMNAF KEITTMADRI NLPRNIVDRT NNLFKQVYEQ KSLKGRANDA, corresponding to N terminal amino acids 109-158 of Human TFIIB

  • 阳性对照
    • Hep G2 cell lysate, human intenstine

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab49135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

WB Use a concentration of 4 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

Anti-TFIIB antibody 图像

  • Anti-TFIIB antibody (ab49135) at 4 µg/ml + HepG2 cell lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling TFIIB with ab49135 at 4-8µg/ml.

Anti-TFIIB antibody (ab49135)参考文献

ab49135 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab49135.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"