Anti-TFII I (phospho Y248)抗体(ab63344)


  • 产品名称Anti-TFII I (phospho Y248)抗体
    参阅全部 TFII I 一抗
  • 描述
    兔多克隆抗体to TFII I (phospho Y248)
  • 特异性ab63344 detects endogenous levels of TFII I only when phosphorylated at tyrosine 248.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    synthesized phosphopeptide derived from human TFII I around the phosphorylation site of tyrosine 248 (P-D-YP-Y-Q).

  • 阳性对照
    • Extracts from LOVO cells



Our Abpromise guarantee covers the use of ab63344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/500.
WB 1/500 - 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).


  • 功能Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
  • 组织特异性Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues.
  • 疾病相关Note=GTF2I is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2I may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • 序列相似性Belongs to the TFII-I family.
    Contains 6 GTF2I-like repeats.
  • 翻译后修饰Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.
  • 细胞定位Cytoplasm. Nucleus. Colocalizes with BTK in the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BAP 135 antibody
    • BAP-135 antibody
    • BAP135 antibody
    • Bruton tyrosine kinase associated protein 135 antibody
    • Bruton tyrosine kinase-associated protein 135 antibody
    • BTK associated protein 135 antibody
    • BTK associated protein 135kD antibody
    • BTK associated protein antibody
    • BTK-associated protein 135 antibody
    • BTKAP 1 antibody
    • BTKAP1 antibody
    • DIWS antibody
    • FLJ38776 antibody
    • FLJ56355 antibody
    • General transcription factor II i antibody
    • General transcription factor II-I antibody
    • General transcription factor IIi antibody
    • GTF 2I antibody
    • Gtf2i antibody
    • GTF2I_HUMAN antibody
    • GTFII I antibody
    • GTFII-I antibody
    • IB 291 antibody
    • IB291 antibody
    • SPIN antibody
    • SRF Phox 1 interacting protein antibody
    • SRF Phox1 interacting protein antibody
    • SRF-Phox1-interacting protein antibody
    • TFII-I antibody
    • Transcription factor II I antibody
    • WBS antibody
    • WBSCR 6 antibody
    • WBSCR6 antibody
    • Williams Beuren syndrome chromosome region 6 antibody
    • Williams Beuren syndrome chromosome region 6 protein antibody
    • Williams-Beuren syndrome chromosomal region 6 protein antibody
    see all

Anti-TFII I (phospho Y248) antibody 图像

  • All lanes : Anti-TFII I (phospho Y248) antibody (ab63344) at 1/500 dilution

    Lane 1 : extracts from LOVO cells, without immunising peptide
    Lane 2 : extracts from LOVO cells, with immunising peptide

    Predicted band size : 112 kDa
    Observed band size : 112 kDa
    Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.

Anti-TFII I (phospho Y248) antibody (ab63344)参考文献

ab63344 has not yet been referenced specifically in any publications.

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Thank you for contacting us. The antibody ab63344 detects TFII I when phosphorylated at tyrosine 248. We do not have a phosphorylated TFII I protein. We do have a ab114787, which is a non-phosphorylated fragment of TFII I, amino acids 1-274, bu...

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Thank you for contacting us and considering the participation in the testing discount scheme. These are the discount codes for the respective antibodies: ab63344 xxxxxxx ab80547 xxxxxxx Both are valid until the xth of xxx 20xx ...

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