概述

  • 产品名称Anti-TFII I抗体
    参阅全部 TFII I 一抗
  • 描述
    兔多克隆抗体to TFII I
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chimpanzee, Rhesus monkey, Orangutan, Bat
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to a region within C terminal residues 950 - 998 of Human TFII I (NP_127492.1)

  • 阳性对照
    • Human: breast adenocarcinoma, prostate adenocarcinoma, human breast carcinoma, colon carcinoma, laryngeal squamous cell carcinoma, linitis plastica stomach cancer, metastatic lymph node, ovarian carcinoma, pancreatic islet cell tumor, skin basal cell carcinoma, skin squamous cell carcinoma, small cell lung cancer, stomach adenocarcinoma, testicular seminoma. Mouse: squamous cell carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab84412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明IHC-P: 1/100 - 1/500. Antigen retrieval with citrate buffer will enhance staining.
    Note: expected to work on frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
    • 组织特异性Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues.
    • 疾病相关Note=GTF2I is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2I may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • 序列相似性Belongs to the TFII-I family.
      Contains 6 GTF2I-like repeats.
    • 翻译后修饰Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.
      Sumoylated.
    • 细胞定位Cytoplasm. Nucleus. Colocalizes with BTK in the cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BAP 135 antibody
      • BAP-135 antibody
      • BAP135 antibody
      • Bruton tyrosine kinase associated protein 135 antibody
      • Bruton tyrosine kinase-associated protein 135 antibody
      • BTK associated protein 135 antibody
      • BTK associated protein 135kD antibody
      • BTK associated protein antibody
      • BTK-associated protein 135 antibody
      • BTKAP 1 antibody
      • BTKAP1 antibody
      • DIWS antibody
      • FLJ38776 antibody
      • FLJ56355 antibody
      • General transcription factor II i antibody
      • General transcription factor II-I antibody
      • General transcription factor IIi antibody
      • GTF 2I antibody
      • Gtf2i antibody
      • GTF2I_HUMAN antibody
      • GTFII I antibody
      • GTFII-I antibody
      • IB 291 antibody
      • IB291 antibody
      • SPIN antibody
      • SRF Phox 1 interacting protein antibody
      • SRF Phox1 interacting protein antibody
      • SRF-Phox1-interacting protein antibody
      • TFII-I antibody
      • Transcription factor II I antibody
      • WBS antibody
      • WBSCR 6 antibody
      • WBSCR6 antibody
      • Williams Beuren syndrome chromosome region 6 antibody
      • Williams Beuren syndrome chromosome region 6 protein antibody
      • Williams-Beuren syndrome chromosomal region 6 protein antibody
      see all

    Anti-TFII I antibody 图像

    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast adenocarcinoma, using 1/100 ab84412 followed by DAB staining.
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Mouse squamous cell carcinoma, using 1/250 ab84412 followed by DAB staining.
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human prostate adenocarcinoma, using 1/250 ab84412 followed by DAB staining.
    • Immunofluorescence analysis of formalin-fixed, paraffin-embedded Human breast carcinoma using 1/100 ab84412 and a Red-fluorescent Alexa Fluor 555 Goat anti-Rabbit IgG at a dilution of 1/500.

    Anti-TFII I antibody (ab84412)参考文献

    ab84412 has not yet been referenced specifically in any publications.

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