概述

  • 产品名称Anti-TFE3抗体
    参阅全部 TFE3 一抗
  • 描述
    兔多克隆抗体to TFE3
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Elephant
  • 免疫原

    Synthetic peptide from a region within amino acids 500-550 of Human TFE3 (NP_006512.2)

  • 阳性对照
    • HeLa and 293T cells

性能

应用

Our Abpromise guarantee covers the use of ab93808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at a concentration of 2-5 µg/mg lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 62 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.
    • 组织特异性Ubiquitous in fetal and adult tissues.
    • 疾病相关Note=A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.
      Note=Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3.
    • 序列相似性Belongs to the MiT/TFE family.
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • 翻译后修饰Sumoylated; does not affect dimerization with MITF.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • bHLH e33 antibody
      • bHLHe33 antibody
      • Class E basic helix-loop-helix protein 33 antibody
      • RCCP 2 antibody
      • RCCP2 antibody
      • RCCX1 antibody
      • Renal cell carcinoma, papillary antibody
      • Tcfe 3 antibody
      • Tcfe3 antibody
      • TFE 3 antibody
      • Tfe3 antibody
      • TFE3_HUMAN antibody
      • TFEA antibody
      • Transcription factor binding to IGHM enhancer 3 antibody
      • Transcription factor E family, member A antibody
      • Transcription factor E3 antibody
      • Transcription factor for IgH enhancer antibody
      • Transcription factor for immunoglobulin heavy chain enhancer 3 antibody
      see all

    Anti-TFE3 antibody 图像

    • All lanes : Anti-TFE3 antibody (ab93808) at 0.04 µg/ml

      Lane 1 : HeLa whole lysate at 50 µg
      Lane 2 : HeLa whole lysate at 15 µg
      Lane 3 : HeLa whole lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 62 kDa


      Exposure time : 3 minutes
    • 1 mg HeLa cell lysate was immunoprecipitated with 3µg/mg ab93808 (lane 1) or control IgG (lane 2). 20% of the immunoprecipitates were analysed by Western blot. Bands were analysed using ab93808 at 0.4µg/ml and developed using cheiluminescence.

    Anti-TFE3 antibody (ab93808)参考文献

    ab93808 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (cardiomyoblast)
    Permeabilization Yes - Triton X 0.5% in PBS
    Specification cardiomyoblast
    Blocking step (agent) for 30 minute(s) · Concentration: 0.5% · Temperature: 24°C
    Fixative Paraformaldehyde
    Username

    Mr. Ahmed Rishiq

    Verified customer

    提交于 May 24 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"