概述

  • 产品名称Anti-TFB1M抗体
    参阅全部 TFB1M 一抗
  • 描述
    小鼠单克隆抗体to TFB1M
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length human TFB1M protein (AAH17788) with a tag.

  • 阳性对照
    • TFB1M transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab77729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 39 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity.
  • 组织特异性Ubiquitously expressed.
  • 疾病相关Note=Variations in TFB1M may influence the clinical expression of aminoglycoside-induced deafness caused by the A1555G mutation in the mitochondrial 12S rRNA.
  • 序列相似性Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.
  • 细胞定位Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • N'-adenosyl(rRNA) dimethyltransferase 1 antibody
    • CGI75 antibody
    • Dimethyladenosine transferase 1, mitochondrial antibody
    • h-mtTFB antibody
    • h-mtTFB1 antibody
    • hmtTFB antibody
    • hmtTFB1 antibody
    • hTFB1M antibody
    • Mitochondrial 12S rRNA dimethylase 1 antibody
    • Mitochondrial dimethyladenosine transferase 1 antibody
    • Mitochondrial transcription factor B1 antibody
    • mtTFB1 antibody
    • S-adenosylmethionine-6-N' antibody
    • Tfb1m antibody
    • TFB1M_HUMAN antibody
    • Transcription factor B1 mitochondrial antibody
    see all

Anti-TFB1M antibody 图像

  • Anti-TFB1M antibody (ab77729) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 39 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-TFB1M antibody (ab77729) at 5 µg/ml

    Lane 1 : TFB1M transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa

Anti-TFB1M antibody (ab77729)参考文献

ab77729 has not yet been referenced specifically in any publications.

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