Anti-TEX19抗体(ab90953)

概述

  • 产品名称Anti-TEX19抗体
    参阅全部 TEX19 一抗
  • 描述
    兔多克隆抗体to TEX19
  • 特异性Immunogen has 86% homology with mouse TEX19 sequence. However, customer feedback indicates that the results for mouse are non-specific (see Abreviews).
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    14 amino acids long synthetic peptide within amino acids 108-157 of Human TEX19

  • 阳性对照
    • Human placenta lysate

性能

应用

Our Abpromise guarantee covers the use of ab90953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-TEX19 antibody 图像

  • Anti-TEX19 antibody (ab90953) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 18 kDa

Anti-TEX19 antibody (ab90953)参考文献

ab90953 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Jurkat cells, Hela, 3T3 NIH, cos1, Testis, ES cell)
Loading amount 50 µg
Specification Jurkat cells, Hela, 3T3 NIH, cos1, Testis, ES cell
Gel Running Conditions Reduced Denaturing (SDS-PAGE gel 12%)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 4% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jan 10 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (16 dpp testes)
Specification 16 dpp testes
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Oct 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"