Anti-Tex14抗体(ab42414)

概述

  • 产品名称Anti-Tex14抗体
    参阅全部 Tex14 一抗
  • 描述
    兔多克隆抗体to Tex14
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Mouse Tex14.

    (Peptide available as ab42413.)

  • 阳性对照
    • In IHC, this antibody gave a positive signal in a mouse testis FFPE section.

性能

应用

Our Abpromise guarantee covers the use of ab42414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 相关性Testis-expressed gene 14 (TEX14) is a novel protein that localizes to germ cell intercellular bridges. In the absence of TEX14, intercellular bridges are not observed. Spermatogenesis in Tex14–/– mice progresses through the transit amplification of diploid spermatogonia and the expression of early meiotic markers but halts before the completion of the first meiotic division.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • Cancer/testis antigen 113 antibody
    • CT113 antibody
    • Inactive serine/threonine protein kinase TEX14 antibody
    • Protein kinase like protein SgK307 antibody
    • Sugen kinase 307 antibody
    • Testis expressed Protein 14 antibody
    • Testis expressed sequence 14 antibody
    • Testis expressed sequence 14 protein antibody
    see all

Anti-Tex14 antibody 图像

  • IHC image of Tex14 staining in mouse normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42414, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Tex14 antibody (ab42414)参考文献

ab42414 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis, postnatal day 6)
Permeabilization Yes - 0.1% Triton X-100 in PBS
Specification Testis, postnatal day 6
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

提交于 Jul 14 2016

Thank you for contacting Abcam. Neither ab42414 or ab41733 have been tested on human samples and based on sequence homology between the mouse immunogen sequence and the human sequence, I do not think that they would react. However, ab74835 will react w...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Testis (20 days post-partum))
Specification Testis (20 days post-partum)
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jan 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"