This fast track antibody is not yet fully characterized. It is subject to these terms and conditions




Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

应用 Ab评论 说明
IHC - Wholemount 1/3000.
ICC/IF 1/400.
IHC-P 1/1000.


  • 相关性Testis-expressed gene 14 (TEX14) is a novel protein that localizes to germ cell intercellular bridges. In the absence of TEX14, intercellular bridges are not observed. Spermatogenesis in Tex14–/– mice progresses through the transit amplification of diploid spermatogonia and the expression of early meiotic markers but halts before the completion of the first meiotic division.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • Cancer/testis antigen 113 antibody
    • CT113 antibody
    • Inactive serine/threonine protein kinase TEX14 antibody
    • Protein kinase like protein SgK307 antibody
    • Sugen kinase 307 antibody
    • Testis expressed Protein 14 antibody
    • Testis expressed sequence 14 antibody
    • Testis expressed sequence 14 protein antibody
    see all

Anti-Tex14 antibody 图像

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • Ab41733 in IHC on PFA fixed adult mouse testis with microwave antigen retrieval. This antibody gave the expected staining pattern of intercellular connections between germ cells (brown dots). It also gave some background staining of sperm tails (in the centre of the tubules). For more information, please see abreview.

    See Abreview

  • This antibody appears to stain intercellular bridges between germ cells, as it should. TEX14 staining is present within the testis cords between between DDX4 positive cells (germ cells), but is absent between DDX4 negative cells (Sertoli cells).
    Postnatal day 6 mouse testes were fixed with 4% paraformaldehyde for 5 hours at 4°C, then washed overnight in PBS at 4°C. Testes were detunicated and cut in half, then permeabilized with 0.1% Triton X-100 in PBS for 30 min at 20°C, then blocked with 3% BSA with 0.1% Triton X-100 in PBS for 1 hour at 20°C. Sections were incubated with Rabbit Anti-Tex14 (Abcam ab41733) diluted 1/3000 plus Goat Anti-DDX4 diluted 1/800 for 14 hours at 4°C. Tissue was then washed 3X for 30 min each with 0.1% Triton X-100 in PBS. Tissue was then incubated with Donkey Anti-Rabbit 555 (Abcam ab150062) plus Donkey Anti-Goat 647 (Abcam ab150155) diluted 1/500 for 3 hours at 20°C. with Abcam DAPI after washing 3X with 0.1% Triton X-100 in PBS. Tissue was then incubated with Fluoroshield Mounting Medium With DAPI (Abcam ab104139) with mounted onto a slide and coverslipped. Tissue was imaged on an Olympus FV 1000 confocal microscope and represented as a maximum intensity Z-projection of a 5 µM thick Z-stack.

    See Abreview

Anti-Tex14 antibody (ab41733)参考文献

This product has been referenced in:
  • Lorès P  et al. Deletion of MgcRacGAP in the male germ cells impairs spermatogenesis and causes male sterility in the mouse. Dev Biol 386:419-27 (2014). Mouse . Read more (PubMed: 24355749) »

See 1 Publication for this product

Product Wall

Application IHC - Wholemount
Sample Mouse Tissue (Testis, postnatal day 6)
Specification Testis, postnatal day 6

Mr. Bryan Niedenberger

Verified customer

提交于 Jul 14 2016

Thank you for calling Abcam earlier today.

I would be happy to issue you a testing discount code to try ab41733 in human samples. Before I do, there is just one further question that I forgot to ask when we talked on the phone. Which instituti...

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Thank you for contacting Abcam. Neither ab42414 or ab41733 have been tested on human samples and based on sequence homology between the mouse immunogen sequence and the human sequence, I do not think that they would react. However, ab74835 will react w...

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Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Sertoli Cell)
Specification Sertoli Cell
Fixative Methanol
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Elad Fadlon

Verified customer

提交于 Jun 25 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Adult testis)
Specification Adult testis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.1M citrate pH6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

提交于 Apr 19 2010