概述

  • 产品名称Anti-TEX12抗体
    参阅全部 TEX12 一抗
  • 描述
    兔多克隆抗体to TEX12
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: MANHLVKPDN RNCKRPRELE SPVPDSPQLS SLGKSDSSFS EISGLFYKDE ALEKDLNDVS, corresponding to amino acids 2-61 of Human TEX12.

  • 阳性对照
    • Human testis tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab122455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/250.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

Anti-TEX12 antibody 图像

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in centrosome. Recommended concentration of ab122455 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1 :
    Lanes 2 - 3 : Anti-TEX12 antibody (ab122455)

    Lane 1 : Marker
    Lane 2 : HEK293T cell lysate - vector only transfected
    Lane 3 : HEK293T cell lysate - overexpressing TEX12 with a C-terminal myc-DDK tag (~3.1kDa)
  • ab122455, at 1/200 dilution, staining TEX12 in paraffin embedded Human testis tissue by Immunohistochemistry.

Anti-TEX12 antibody (ab122455)参考文献

ab122455 has not yet been referenced specifically in any publications.

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