兔多克隆抗体to Tetraspanin 9
This antibody is specific for tetraspanin Tspan 9.
Mouse, Rat, Human
, corresponding to C terminal amino acids 229-239 of Human Tetraspanin 9
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.1% Sodium Azide
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at a concentration of 1 µg.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 27 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Expressed in megakaryocytes and platelets (at protein level).
Belongs to the tetraspanin (TM4SF) family.
Membrane. Colocalizes with GP6 in tetraspanin microdomains on the platelet surface.
Information by UniProt
Western blot - Anti-Tetraspanin 9 antibody (ab61261)
All lanes :
Anti-Tetraspanin 9 antibody (ab61261) at 1 µg/ml
Lane 1 :
Lane 2 :
Performed under reducing conditions.
Predicted band size:
Observed band size:
19,23,26 kDa (
why is the actual band size different from the predicted?
Whole cell lysates of transfected cells were used.
Blocking buffer 5% skimmed milk, incubation time 1-2 hours, exposure time 5 minutes, 13% SDS-PAGE gel.
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