Anti-Tetraspanin 9抗体(ab43620)

概述

  • 产品名称
    Anti-Tetraspanin 9抗体
    参阅全部 Tetraspanin 9 一抗
  • 描述
    小鼠多克隆抗体to Tetraspanin 9
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment:

    RSFSRLRRNPQSADSGADTSGRRDSADKC

    , corresponding to amino acids 23-51 of Human Tetraspanin 9

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度
    Whole antiserum
  • Primary antibody说明
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab43620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 27 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

IHC-P Use at an assay dependent concentration. PubMed: 14145

靶标

  • 组织特异性
    Expressed in megakaryocytes and platelets (at protein level).
  • 序列相似性
    Belongs to the tetraspanin (TM4SF) family.
  • 翻译后修饰
    Glycosylated.
  • 细胞定位
    Membrane. Colocalizes with GP6 in tetraspanin microdomains on the platelet surface.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 6720474K14Rik antibody
    • 9430079M16Rik antibody
    • AU018597 antibody
    • NET 5 antibody
    • NET5 antibody
    • New EST tetraspan 5 antibody
    • PP1057 antibody
    • Tetraspan NET 5 antibody
    • Tetraspan NET-5 antibody
    • Tetraspanin-9 antibody
    • Transmembrane 4 superfamily member tetraspan NET 5 antibody
    • TSN9_HUMAN antibody
    • Tspan-9 antibody
    • TSPAN9 antibody
    see all

Anti-Tetraspanin 9 antibody (ab43620)参考文献

ab43620 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Lung)
Loading amount
20 µg
Specification
Lung
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

提交于 Jun 04 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Normal liver)
Specification
Normal liver
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: edta
Permeabilization
No
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

提交于 Apr 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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