Anti-Tet2抗体(ab99432)

概述

  • 产品名称Anti-Tet2抗体
    参阅全部 Tet2 一抗
  • 描述
    山羊多克隆抗体to Tet2
  • 特异性This antibody is expected to recognize both reported isoforms of Human TET2(NP_001120680.1; NP_060098.3).
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Dog
  • 免疫原

    Synthetic peptide corresponding to internal amino acids 761-775 (

    C-PHPQSNNDQQREGSF

    ) of both isoforms of Human TET2 (NP_001120680.1; NP_060098.3).

  • 阳性对照
    • Human brain lysates (Amygdala and Hippocampus) Human spleen.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab99432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2 - 6 µg/ml.

In paraffin-embedded Human Spleen shows nuclear staining in select splenocytes.

Never incubate primary overnight. We know a lot of customers get non-specific background after overnight incubations. We always recommend to incubate for 1h at room temp. This allows a higher signal/background ratio at better controlled conditions.

WB Use a concentration of 0.3 µg/ml. Predicted molecular weight: 130 kDa.

Preliminary experiments gave an approx 80kDa band in Human Brain (Amygdala and Hippocampus) lysates after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 130kDa according to NP_060098.3. The 80kDa band was successfully blocked by incubation with the immunizing peptide.

Never incubate primary overnight. We know a lot of customers get non-specific background after overnight incubations. We always recommend to incubate for 1h at room temp. This allows a higher signal/background ratio at better controlled conditions.

靶标

  • 功能Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). Plays an important role in myelopoiesis. The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation.
  • 组织特异性Broadly expressed. Highly expressed in hematopoietic cells; highest expression observed in granulocytes. Expression is reduced in granulocytes from peripheral blood of patients affected by myelodysplastic syndromes.
  • 疾病相关Note=TET2 is frequently mutated in myeloproliferative disorders (MPD). These constitute a heterogeneous group of disorders, also known as myeloproliferative diseases or myeloproliferative neoplasms (MPN), characterized by cellular proliferation of one or more hematologic cell lines in the peripheral blood, distinct from acute leukemia. Included diseases are: essential thrombocythemia, polycythemia vera, primary myelofibrosis (chronic idiopathic myelofibrosis). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites.
    Defects in TET2 are a cause of polycythemia vera (PV) [MIM:263300]. A myeloproliferative disorder characterized by abnormal proliferation of all hematopoietic bone marrow elements, erythroid hyperplasia, an absolute increase in total blood volume, but also by myeloid leukocytosis, thrombocytosis and splenomegaly.
    Note=TET2 is frequently mutated in systemic mastocytosis; also known as systemic mast cell disease. A condition with features in common with myeloproliferative diseases. It is a clonal disorder of the mast cell and its precursor cells. The clinical symptoms and signs of systemic mastocytosis are due to accumulation of clonally derived mast cells in different tissues, including bone marrow, skin, the gastrointestinal tract, the liver, and the spleen.
    Note=TET2 is frequently mutated in myelodysplastic syndromes, a heterogeneous group of closely related clonal hematopoietic disorders. All are characterized by a hypercellular or hypocellular bone marrow with impaired morphology and maturation, dysplasia of the myeloid, megakaryocytic and/or erythroid lineages, and peripheral blood cytopenias resulting from ineffective blood cell production. Included diseases are: refractory anemia (RA), refractory anemia with ringed sideroblasts (RARS), refractory anemia with excess blasts (RAEB), refractory cytopenia with multilineage dysplasia and ringed sideroblasts (RCMD-RS). Chronic myelomonocytic leukemia (CMML) is a myelodysplastic/myeloproliferative disease. Myelodysplastic syndromes are considered a premalignant condition in a subgroup of patients that often progresses to acute myeloid leukemia (AML). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites.
  • 序列相似性Belongs to the TET family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ20032 antibody
    • KIAA1546 antibody
    • MDS antibody
    • Methylcytosine dioxygenase TET2 antibody
    • Nbla00191 antibody
    • Probable methylcytosine dioxygenase TET2 antibody
    • Protein Ayu17 449 antibody
    • Tet 2 antibody
    • Tet methylcytosine dioxygenase 2 antibody
    • Tet oncogene 2 antibody
    • Tet oncogene family member 2 antibody
    • TET2 antibody
    • TET2_HUMAN antibody
    see all

Anti-Tet2 antibody 图像

  • Immunohistochemical staining of paraffin embedded human spleen with ab99432 at 4 μg/mL. Heat mediated antigen retrieval was performed with citrate buffer, pH 6.

Anti-Tet2 antibody (ab99432)参考文献

ab99432 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. I am pleased to provide discount codes for the customer to test ab94580 and ab99432 in mouse. The alignment of the immunogen with the mouse protein for both antibodies is high, so they are likely to detect in mouse. Howe...

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