概述

  • 产品名称Anti-Tet2抗体
    参阅全部 Tet2 一抗
  • 描述
    兔多克隆抗体to Tet2
  • 经测试应用适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide corresponding to Mouse Tet2 aa 1600-1700 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q4JK59

  • 阳性对照
    • This antibody gave a positive signal in the following Mouse whole cell lysates: E14Tg2A ; Wild Type Mouse ES Cell Lysate (Tet2 Positive Control)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab124297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/250. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
IP Use at an assay dependent concentration.

靶标

  • 功能Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). Plays an important role in myelopoiesis. The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation.
  • 组织特异性Broadly expressed. Highly expressed in hematopoietic cells; highest expression observed in granulocytes. Expression is reduced in granulocytes from peripheral blood of patients affected by myelodysplastic syndromes.
  • 疾病相关Note=TET2 is frequently mutated in myeloproliferative disorders (MPD). These constitute a heterogeneous group of disorders, also known as myeloproliferative diseases or myeloproliferative neoplasms (MPN), characterized by cellular proliferation of one or more hematologic cell lines in the peripheral blood, distinct from acute leukemia. Included diseases are: essential thrombocythemia, polycythemia vera, primary myelofibrosis (chronic idiopathic myelofibrosis). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites.
    Defects in TET2 are a cause of polycythemia vera (PV) [MIM:263300]. A myeloproliferative disorder characterized by abnormal proliferation of all hematopoietic bone marrow elements, erythroid hyperplasia, an absolute increase in total blood volume, but also by myeloid leukocytosis, thrombocytosis and splenomegaly.
    Note=TET2 is frequently mutated in systemic mastocytosis; also known as systemic mast cell disease. A condition with features in common with myeloproliferative diseases. It is a clonal disorder of the mast cell and its precursor cells. The clinical symptoms and signs of systemic mastocytosis are due to accumulation of clonally derived mast cells in different tissues, including bone marrow, skin, the gastrointestinal tract, the liver, and the spleen.
    Note=TET2 is frequently mutated in myelodysplastic syndromes, a heterogeneous group of closely related clonal hematopoietic disorders. All are characterized by a hypercellular or hypocellular bone marrow with impaired morphology and maturation, dysplasia of the myeloid, megakaryocytic and/or erythroid lineages, and peripheral blood cytopenias resulting from ineffective blood cell production. Included diseases are: refractory anemia (RA), refractory anemia with ringed sideroblasts (RARS), refractory anemia with excess blasts (RAEB), refractory cytopenia with multilineage dysplasia and ringed sideroblasts (RCMD-RS). Chronic myelomonocytic leukemia (CMML) is a myelodysplastic/myeloproliferative disease. Myelodysplastic syndromes are considered a premalignant condition in a subgroup of patients that often progresses to acute myeloid leukemia (AML). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites.
  • 序列相似性Belongs to the TET family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ20032 antibody
    • KIAA1546 antibody
    • MDS antibody
    • Methylcytosine dioxygenase TET2 antibody
    • Nbla00191 antibody
    • Probable methylcytosine dioxygenase TET2 antibody
    • Protein Ayu17 449 antibody
    • Tet 2 antibody
    • Tet methylcytosine dioxygenase 2 antibody
    • Tet oncogene 2 antibody
    • Tet oncogene family member 2 antibody
    • TET2 antibody
    • TET2_HUMAN antibody
    see all

Anti-Tet2 antibody 图像

  • ICC/IF image of Anti-Tet2 antibody (ab124297) stained WT and Tet2-/- mouse ES cells. The cells were fixed in PFA, permeabilized using 0.25% Triton X-100, and blocked with 10% BSA for 30 minutes. The cells were then incubated with ab124297 at a 1/400 dilution for 13 hours and 20 minutes at 4oC. The secondary antibody was a Rhodamine Red-X AffiniPure Donkey anti-Rabbit used at a 1/500 dilution.

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  • All lanes : Anti-Tet2 antibody (ab124297) at 1/500 dilution

    Lane 1 : Wild-type embryonic stem cells
    Lane 2 : Tet2-/- embryonic stem cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat anti-Rabbit (whole molecules) IgG HRP at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 223 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an anonymous abreview.

    See Abreview

  • All lanes : Anti-Tet2 antibody (ab124297) at 1/250 dilution (Milk blocking - 1%)

    Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
    Lane 2 : WT Mouse ES Cell Lysate (Positive Control for Tet2)
    Lane 3 : Tet2 Knockout Mouse ES Cell Lysate (Negative Control)

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 223 kDa
    Observed band size : 223 kDa
    Additional bands at : 48 kDa (possible non-specific binding),65 kDa (possible non-specific binding),70 kDa (possible non-specific binding).

    Exposure time : 4 minutes

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk before being incubated with ab124297 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

     

    Secondary antibody - goat anti-rabbit HRP (ab97051)

  • ICC/IF image of Anti-Tet2 antibody (ab124297) stained D3 mouse ES cells. The cells were fixed in 4% PFA, permeabilized using 0.1% Triton X-100, and blocked with 1% Goat serum, 0.1% BSA in PBS for 30 minutes. The cells were then incubated with ab124297 at a 1/100 dilution for 2 hours at RT. The secondary antibody was a Goat polyclonal Secondary Antibody to Rabbit IgG – H&L Alexa Fluor 488 (ab150077) used at a 1/200 dilution.

     

    Secondary antibody - goat anti-rabbit Alexa  488 (ab150077)

    See Abreview

  • Immunoprecipitation of Tet2, using WT and Tet2 KO mouse ES cells. The protein was immunoprecipitated using 300µg whole cell extract, and 3µg of Rabbit polyclonal to Tet2 (ab124297). The same Tet2 antibody was used for the western blot. Tet2 KO mouse ES cells were used as a negative control.

    Band: 223kDa: Tet2.

    See Abreview

Anti-Tet2 antibody (ab124297)参考文献

This product has been referenced in:
  • Huang Y  et al. Distinct roles of the methylcytosine oxidases Tet1 and Tet2 in mouse embryonic stem cells. Proc Natl Acad Sci U S A 111:1361-6 (2014). Mouse . Read more (PubMed: 24474761) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Pancreas)
Permeabilization Yes - 1ml 0.15% Triton-X-100 in 1 x PBS
Specification Pancreas
Blocking step NDS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Paraformaldehyde
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Abcam user community

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提交于 Jun 27 2016

Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (Murine embryonic stem cells)
Total protein in input 15 µg
Immuno-precipitation step Protein A/G
Specification Murine embryonic stem cells
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提交于 Nov 09 2015

Application Western blot
Sample Mouse Cell lysate - whole cell (embryonic stem cells)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Gel)
Loading amount 20 µg
Specification embryonic stem cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Nov 08 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Sample Mouse Cell (Embryonic Stem Cells)
Specification Embryonic Stem Cells
Permeabilization Yes - 0.25% Triton X-100
Fixative Paraformaldehyde
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提交于 Oct 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Specification mouse embryonic stem cells
Total protein in input 300 µg
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提交于 Aug 20 2013

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Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 21°C
Sample Mouse Cell (D3 Mouse Embryonic Stem Cell line)
Specification D3 Mouse Embryonic Stem Cell line
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
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Joe Segal

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提交于 Aug 14 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (embryonic stem cells)
Specification embryonic stem cells
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Abcam user community

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提交于 Aug 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Specification mouse embryonic stem cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Jul 29 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"