Testosterone ELISA试剂盒(ab108666)
Key features and details
- Sensitivity: 0.07 ng/ml
- Range: 0.2 ng/ml - 16 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
概述
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产品名称
Testosterone ELISA试剂盒
参阅全部 Testosterone 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Control Sera 48 <=5.8% 批次间 样品 n Mean SD CV% Control Sera 48 <=10.5% -
样品类型
Urine, Serum, Hep Plasma, Cit plasma -
检测类型
Competitive -
灵敏度
= 0.07 ng/ml -
范围
0.2 ng/ml - 16 ng/ml -
回收率
= 105.5 %
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实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Testosterone ELISA kit is a competitive ELISA designed for the accurate quantitative measurement of Testosterone in serum and plasma.
A 96-well plate has been precoated with anti-Testosterone antibodies. Samples and the Testosterone-HRP conjugate are added to the wells, where Testosterone in the sample competes with the added Testosterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Testosterone in the sample and the intensity is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Wash Buffer 1 x 50ml Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit Testosterone conjugate 1 x 12ml Testosterone Control A 1 x 1ml Testosterone Control B 1 x 1ml Testosterone IgG Coated Microtiter Plate (12 x 8 wells) 1 unit Testosterone Standard 0 - 0.0 ng/mL 1 x 1ml Testosterone Standard 1 - 0.6 ng/mL 1 x 1ml Testosterone Standard 2 - 1.4 ng/mL 1 x 1ml Testosterone Standard 3 - 4.0 ng/mL 1 x 1ml Testosterone Standard 4 - 16.0 ng/mL 1 x 1ml TMB Substrate Solution 1 x 15ml -
研究领域
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别名
- 17ß-hydroxyandrost-4-ene-3-one
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数据库链接
- SwissProt: P04278 Human
图片
数据表及文件
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SDS download
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Datasheet download
文献 (52)
ab108666 被引用在 52 文献中.
- Kelava I et al. Androgens increase excitatory neurogenic potential in human brain organoids. Nature 602:112-116 (2022). PubMed: 35046577
- Chi A et al. ICA II Alleviates Testicular Torsion Injury by Dampening the Oxidative and Inflammatory Stress. Front Endocrinol (Lausanne) 13:871548 (2022). PubMed: 35634492
- Wen M et al. CTBP1‑AS upregulation is associated with polycystic ovary syndrome and can be effectively downregulated by cryptotanshinone. Mol Med Rep 26:N/A (2022). PubMed: 35656892
- Zheng B et al. Testosterone protects mice against zika virus infection and suppresses the inflammatory response in the brain. iScience 25:105300 (2022). PubMed: 36304103
- El-Khadragy MF et al. Chlorogenic acid abates male reproductive dysfunction in arsenic-exposed mice via attenuation of testicular oxido-inflammatory stress and apoptotic responses. Chem Biol Interact 333:109333 (2021). PubMed: 33242462