概述

  • 产品名称Anti-Testin抗体
    参阅全部 Testin 一抗
  • 描述
    小鼠单克隆抗体to Testin
  • 经测试应用适用于: WB, ICC/IF, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Chicken, Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-422 of Human Testin

性能

应用

Our Abpromise guarantee covers the use of ab57292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 48 kDa.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Scaffold protein that may play a role in cell adhesion, cell spreading and in the reorganization of the actin cytoskeleton. Plays a role in the regulation of cell proliferation. May act as a tumor suppressor. Inhibits tumor cell growth.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the prickle / espinas / testin family.
    Contains 3 LIM zinc-binding domains.
    Contains 1 PET domain.
  • 结构域The N-terminal and the C-terminal halves of the protein can associate with each other, thereby hindering interactions with ZYX.
  • 细胞定位Cytoplasm. Cell junction > focal adhesion. Detected along actin stress fibers.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZP586B2022 antibody
    • MGC1146 antibody
    • TES antibody
    • TES_HUMAN antibody
    • TESS 2 antibody
    • TESS antibody
    • TESS2 antibody
    • Testin antibody
    • Testis derived transcript (3 LIM domains) antibody
    • Testis derived transcript antibody
    see all

Anti-Testin antibody 图像

  • Overlay histogram showing HeLa cells stained with ab57292 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57292, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Testin antibody (ab57292) used in immunofluorescence at 10ug/ml on HeLa cells.
  • Testin antibody (ab57292) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil tissue.


  • Predicted band size : 48 kDa
    Testin antibody (ab57292) at 1ug/lane + K-562 cell lysate at 25ug/lane.

Anti-Testin antibody (ab57292)参考文献

This product has been referenced in:
  • Gu Z  et al. TESTIN suppresses tumor growth and invasion via manipulating cell cycle progression in endometrial carcinoma. Med Sci Monit 20:980-7 (2014). IHC-P ; Human . Read more (PubMed: 24929083) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Chicken Tissue sections (Neuronal)
Specification Neuronal
Fixative Methanol
Permeabilization Yes - 0.1% Tween 20 in PBS
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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提交于 Apr 16 2012

DISCOUNT CODE: Expiration date: 14 May 2012 I am very pleased to hear you would like to accept our offer and test ab57292 in chicken. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abre...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"