RabMAb

Anti-Tenascin C抗体[EPR4219] (ab108930)

概述

  • 产品名称Anti-Tenascin C抗体[EPR4219]
    参阅全部 Tenascin C 一抗
  • 描述
    兔单克隆抗体[EPR4219] to Tenascin C
  • 特异性IHC on human tissues which we tested (such as testis, pancreas and stomach) showed non-specific staining. We don’t recommend this antibody for IHC on human tissues.
  • 经测试应用适用于: WB, IHC-P, IHC-Frmore details
    不适用于: ICC or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Tenascin C.

  • 阳性对照
    • Human fetal brain lysate, U87-MG (human glioblastoma) whole cell lysate, mouse cerebellum, mouse cerebellar cortex and rat cerebellar cortex.
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab108930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 241 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 24384131

Antigen retrieval is recommended.

IHC-Fr Use at an assay dependent concentration.
  • 应用说明Is unsuitable for ICC or IP.
  • 靶标

    • 功能Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
    • 序列相似性Belongs to the tenascin family.
      Contains 15 EGF-like domains.
      Contains 1 fibrinogen C-terminal domain.
      Contains 15 fibronectin type-III domains.
    • 细胞定位Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 150 225 antibody
      • Cytotactin antibody
      • Glioma associated extracellular matrix antigen antibody
      • Glioma-associated-extracellular matrix antigen antibody
      • GMEM antibody
      • GP 150 225 antibody
      • GP 150-225 antibody
      • GP antibody
      • Hexabrachion antibody
      • HXB antibody
      • JI antibody
      • MGC167029 antibody
      • Miotendinous antigen antibody
      • Myotendinous antigen antibody
      • Neuronectin antibody
      • TENA_HUMAN antibody
      • Tenascin antibody
      • Tenascin-C antibody
      • Tenascin-C isoform 14/AD1/16 antibody
      • TenascinC antibody
      • TN antibody
      • TN C antibody
      • TN-C antibody
      • TNC antibody
      see all

    Anti-Tenascin C antibody [EPR4219] 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA Buffer, PH9 (ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counter stain. Staining on the molecular layer of rat cerebellar cortex (PMID: 1372043).

    • Immunohistochemistry (Frozen sections) analysis of Mouse E14 spinal cord labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilised with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, an AlexaFluor® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counter stain. Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID: 9822997; PMID: 19586317; PMID: 24778247).

    • Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution + U87-MG (human glioblastoma) whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size : 241 kDa
      Observed band size : 250 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and diluting buffer and concentration: 5% NFDM /TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).

    • Immunohistochemistry (Frozen sections) analysis of Rat cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilised with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, an AlexaFluor® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counter stain. Positive staining on the molecular layer of rat cerebellar cortex (PMID: 1372043).

    • Immunohistochemistry (Frozen sections) analysis of Mouse E14 cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilised with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, an AlexaFluor® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counter stain. Positive staining on the molecular layer of mouse E14 cerebellar cortex (PMID: 1372043).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse E14 spinal cord tissue sections labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer, PH9 (ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counter stain. Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID: 9822997; PMID: 19586317; PMID: 24778247).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer, PH9 (ab93684). Hematoxylin was used to counter stain. A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Staining on the molecular layer of mouse cerebellar cortex (PMID: 1372043).

    • All lanes : Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution

      Lane 1 : Postnatal (P0) Mouse cerebellum
      Lane 2 : Postnatal (P0) Rat brain

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 241 kDa
      Observed band size : 250 kDa (why is the actual band size different from the predicted?)

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure Time:
      Lane 1: 15 seconds
      Lane 2: 30 seconds

      The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).

    • All lanes : Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution

      Lane 1 : Human fetal brain
      Lane 2 : Human liver

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size : 241 kDa
      Observed band size : 250 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and diluting buffer and concentration: 5% NFDM /TBST.

      Liver is negative control (PMID: 1717349). The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).

    • Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution + Human fetal brain lysate at 10 µg

      Predicted band size : 241 kDa

    Anti-Tenascin C antibody [EPR4219] (ab108930)参考文献

    This product has been referenced in:
    • Fu H  et al. Tenascin-C Is a Major Component of the Fibrogenic Niche in Kidney Fibrosis. J Am Soc Nephrol N/A:N/A (2016). Read more (PubMed: 27612995) »
    • Govindarajan P  et al. Bone matrix, cellularity, and structural changes in a rat model with high-turnover osteoporosis induced by combined ovariectomy and a multiple-deficient diet. Am J Pathol 184:765-77 (2014). IHC ; Rat . Read more (PubMed: 24384131) »

    See all 4 Publications for this product

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    All of our Western Blots are tested under reducing and denaturing conditions unless noted on the datasheet.

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    Read More
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Jaculus jaculus Tissue sections (Foot section/joint)
    Antigen retrieval step Enzymatic - Buffer/Enzyme Used: ProteaseK
    Permeabilization No
    Specification Foot section/joint
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Mai Tran

    Verified customer

    提交于 Jul 12 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (skin)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
    Permeabilization No
    Specification skin
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative 10% buffered formalin
    Username

    Abcam user community

    Verified customer

    提交于 May 09 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (pancreatic tumor)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate pH 6.0
    Permeabilization No
    Specification pancreatic tumor
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative 10% buffered formalin
    Username

    Abcam user community

    Verified customer

    提交于 Sep 25 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (breast tumor)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
    Permeabilization No
    Specification breast tumor
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative 10% buffered formalin
    Username

    Abcam user community

    Verified customer

    提交于 Sep 25 2015

    Application Immunohistochemistry (Frozen sections)
    Sample Cow Tissue sections (Tendon)
    Permeabilization No
    Specification Tendon
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    提交于 Sep 21 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (MSC)
    Permeabilization Yes - Triton 1%
    Specification MSC
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Apr 29 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step FBS / BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9
    Sample Human Tissue sections (Infantile fibromatosis)
    Specification Infantile fibromatosis
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jul 16 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample Human Cell (293T)
    Specification 293T
    Permeabilization Yes - Triton X-100 1%
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jun 04 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9
    Sample Mouse Tissue sections (Mouse 15.5dpc)
    Specification Mouse 15.5dpc
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jan 23 2014

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