概述

  • 产品名称
    Anti-TEM8抗体[200C1339]
    参阅全部 TEM8 一抗
  • 描述
    小鼠单克隆抗体[200C1339] to TEM8
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    CINFTRVKNNQPAKYPL

    , corresponding to amino acids 481-497 of Human TEM8.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Tested with TEM8 transfected cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab13798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 84 kDa.

A 70 kD band was seen when cell extracts were treated with a glycosidase cocktail. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
IHC-P 1/200 - 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells.
  • 组织特异性
    Detected in umbilical vein endothelial cells (at protein level). Highly expressed in tumor endothelial cells.
  • 疾病相关
    Defects in ANTXR1 are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • 序列相似性
    Belongs to the ATR family.
    Contains 1 VWFA domain.
  • 结构域
    Binding to PA occurs through the VWA domain.
  • 细胞定位
    Cell membrane. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. At the membrane of lamellipodia and at the tip of actin-enriched filopodia. Colocalizes with actin at the base of lamellipodia.
  • Information by UniProt
  • 数据库链接
    • 别名
      • Anthrax toxin receptor 1 antibody
      • ANTR1_HUMAN antibody
      • Antxr1 antibody
      • ATR antibody
      • Tumor endothelial marker 8 antibody
      see all

    图片

    • Western blot - Anti-TEM8 antibody [200C1339] (ab13798)
      Western blot - Anti-TEM8 antibody [200C1339] (ab13798)

      Western blot detection of TEM8 in TEM8 transfected cell lysate using ab13798 at 1 µg/ml.

      Western blot detection of TEM8 in TEM8 transfected cell lysate using ab13798 at 1 µg/ml.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8 antibody [200C1339] (ab13798)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8 antibody [200C1339] (ab13798)
      Formalin-fixed, paraffin-embedded human colon stained with ab13798 diluted 1:200, detection using peroxidase-conjugated secondary antibody and DAB chromogen.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8 antibody [200C1339] (ab13798)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8 antibody [200C1339] (ab13798)
      Formalin-fixed, paraffin-embedded human colon stained with ab13798 diluted 1:200, detection using peroxidase-conjugated secondary and DAB chromogen.

    文献

    This product has been referenced in:
    • Sakai Y  et al. Bcl-2 mediated modulation of vascularization in prostate cancer xenografts. Prostate 69:459-70 (2009). WB ; Human . Read more (PubMed: 19107861) »

    See 1 Publication for this product

    发表研究结果有使用 ab13798？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    1 Review or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-TEM8 antibody [200C1339]

     Good
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (Hemangiosarcoma cell lines)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    50 µg
    Specification
    Hemangiosarcoma cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    abreview image
    Username

    Holly Pondenis

    Verified customer

    提交于 Feb 20 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册