Anti-TEM7抗体[197C193] (ab13667)

概述

  • 产品名称
    Anti-TEM7抗体[197C193]
    参阅全部 TEM7 一抗
  • 描述
    小鼠单克隆抗体[197C193] to TEM7
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide:

    NNLSPKTKGTPVHLGTI

    , corresponding to amino acids 411-427 of Human TEM7.

  • 阳性对照
    • HCT-116 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab13667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
WB
  • 应用说明
    WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.
    Used at a dilution of 1/1500 for 16 hrs on human brain tumor (see Abreview for further information).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Plays a critical role in endothelial cell capillary morphogenesis.
    • 组织特异性
      Detected in endothelial cells from colorectal cancer, and in endothelial cells from primary cancers of the lung, liver, pancreas, breast and brain. Not detectable in endothelial cells from normal tissue. Expressed in fibrovascular membrane with increased expression in individuals with proliferative diabetic retinopathy.
    • 序列相似性
      Belongs to the plexin family.
    • 翻译后修饰
      N-glycosylated.
    • 细胞定位
      Cytoplasm; Secreted and Cell membrane. Cell junction > tight junction. Localized predominantly at the tight junctions of vascular endothelial cells and to a lesser extent at the luminal surface of vascular endothelial cells.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp686F0937 antibody
      • FLJ36270 antibody
      • FLJ45632 antibody
      • OTTHUMP00000164240 antibody
      • Plexin domain containing 1 antibody
      • Plexin domain containing protein 1 precursor antibody
      • Plexin domain-containing protein 1 antibody
      • Plxdc1 antibody
      • PXDC1_HUMAN antibody
      • TEM3 antibody
      • TEM7 antibody
      • Tumor endothelial marker 3 antibody
      • Tumor endothelial marker 7 antibody
      see all

    Anti-TEM7 antibody [197C193] 图像

    • ab13667 at a 1/1500 dilution staining human brain tumor (glioblatoma) samples by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Heat mediated antigen retrieval was used and the tissue was fixed with ethanol and blocked with 2.5% serum prior to incubation with the antibody for 16 hours. A pan-specifc universal secondary antibody was used.

      See Abreview

    Anti-TEM7 antibody [197C193] (ab13667)参考文献

    ab13667 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Brain tumor)
    Specification
    Brain tumor
    Fixative
    Acetone
    Antigen retrieval step
    Heat mediated
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2.5
    Username

    Abcam user community

    Verified customer

    提交于 Jul 13 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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