Anti-TEKT2抗体(ab60918)

概述

  • 产品名称Anti-TEKT2抗体
    参阅全部 TEKT2 一抗
  • 描述
    小鼠单克隆抗体to TEKT2
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Partial recombinant fragment: MATLSVKPSR RFQLPDWHTN SYLLSTNAQL QRDASHQIRQ EARVLRNETN NQTIWDEHDN RTRLVERIDT VNRWKEMLDK CLTDLDAEID ALTQMKESA, corresponding to amino acids 1-99 of TEKT2, with a tag

性能

应用

Our Abpromise guarantee covers the use of ab60918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 50 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Structural component of ciliary and flagellar microtubules. Plays a key role in the assembly or attachment of the inner dynein arm to microtubules in sperm flagella and tracheal cilia. Forms filamentous polymers in the walls of ciliary and flagellar microtubules.
  • 组织特异性Expressed at high levels in testis, trachea and fetal lung, and at lower levels in ovary, pituitary, adult lung, fetal brain and fetal kidney.
  • 序列相似性Belongs to the tektin family.
  • 翻译后修饰Tyrosine phosphorylated.
  • 细胞定位Cell projection > cilium > flagellum. Cytoplasm > cytoskeleton > cilium axoneme. Cytoplasm > cytoskeleton > flagellum axoneme. In sperm, observed in a discontinuous punctate pattern in the flagellum and in the postacrosomal head region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • TEKT2 antibody
    • TEKT2_HUMAN antibody
    • TEKTB1 antibody
    • Tektin 2 antibody
    • Tektin B1 antibody
    • Tektin t antibody
    • Tektin-2 antibody
    • Tektin-B1 antibody
    • Tektin-t antibody
    • Testicular tektin antibody
    • Testicular tektin B1 like protein antibody
    • Testicular tektin B1-like protein antibody
    see all

Anti-TEKT2 antibody 图像

  • Anti-TEKT2 antibody (ab60918) + immunogen

    Predicted band size : 50 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab60918 staining human Hela cells by ICC/IF. Cells were methanol or formaldehyde fixed and not permeabilized prior to blocking in 2% BSA for 30 minutes. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour. The secondary antibody was Alexa Fluor® 594 conjugated donkey polyclonal to mouse, diluted 1/1000.

    See Abreview

Anti-TEKT2 antibody (ab60918)参考文献

ab60918 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2%
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提交于 Nov 27 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"