概述

  • 产品名称Anti-TECK抗体
    参阅全部 TECK 一抗
  • 描述
    兔多克隆抗体to TECK
  • 经测试应用适用于: WB, ELISA, Neutralising, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Highly pure (>98%) recombinant hTECK (human Thymus Expressed Chemokine)

性能

  • 形式Lyophilised:Reconstitute with 200µl of sterile water.
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型unknown
  • 轻链类型unknown
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab2164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hTECK by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTECK.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hTECK (100.00 ng/ml), a concentration of 10.0 - 15.0 µg/ml of this antibody is required.
Sandwich ELISA Use a concentration of 0.5 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to TECK (Biotin) (ab83146).

Assuming 100µl/well, a concentration of 0.5-2.0 µg/ml of ab2164 will detect at least 0.2-0.4 ng/well of recombinant human TECK when used with ab83146 as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.

靶标

  • 功能Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1.
  • 组织特异性Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
  • 序列相似性Belongs to the intercrine beta (chemokine CC) family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A130072A22Rik antibody
    • C-C motif chemokine 25 antibody
    • CCL 25 antibody
    • CCL25 antibody
    • CCL25_HUMAN antibody
    • Chemokine (C C motif) ligand 25 antibody
    • Chemokine (CC motif) ligand 25 antibody
    • Chemokine C C motif ligand 25 antibody
    • Chemokine CC motif ligand 25 antibody
    • Chemokine TECK antibody
    • Ck beta 15 antibody
    • Ckb 15 antibody
    • Ckb15 antibody
    • MGC125074 antibody
    • MGC150327 antibody
    • SCY A25 antibody
    • SCYA 25 antibody
    • SCYA25 antibody
    • Small inducible cytokine A25 antibody
    • Small inducible cytokine A25 isoform 1 precursor antibody
    • Small inducible cytokine A25 isoform 2 precursor antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 25 antibody
    • Small inducible cytokine subfamily A Cys Cys member 25 antibody
    • Small inducible cytokine subfamily A, member 25 antibody
    • Small-inducible cytokine A25 antibody
    • TECK antibody
    • TECKvar antibody
    • Thymus expressed chemokine antibody
    • Thymus-expressed chemokine antibody
    see all

Anti-TECK antibody (ab2164)参考文献

ab2164 has not yet been referenced specifically in any publications.

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