Anti-TEA domain family member 2/ETF抗体(ab92279)
Key features and details
- Goat polyclonal to TEA domain family member 2/ETF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TEA domain family member 2/ETF抗体
参阅全部 TEA domain family member 2/ETF 一抗 -
描述
山羊多克隆抗体to TEA domain family member 2/ETF -
宿主
Goat -
特异性
Not expected to cross-react with other TEA domain family proteins. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Dog -
免疫原
Synthetic peptide corresponding to Human TEA domain family member 2/ETF aa 238-249 (internal sequence).
Sequence:C-EPPDAVDSYQRH
Database link: NP_003589.1 -
阳性对照
- Human ileum tissue lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92279于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif. -
序列相似性
Contains 1 TEA DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8463 Human
- Entrez Gene: 21677 Mouse
- Entrez Gene: 308582 Rat
- Omim: 601729 Human
- SwissProt: Q15562 Human
- SwissProt: P48301 Mouse
- Unigene: 515534 Human
- Unigene: 3019 Mouse
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别名
- Embryonic TEA domain containing factor antibody
- Etdf antibody
- ETEF 1 antibody
see all
图片
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Anti-TEA domain family member 2/ETF antibody (ab92279) at 1 µg/ml + Human colon lysate (35µg protein in RIPA buffer)
Predicted band size: 49 kDaDetected by chemiluminescence.
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Anti-TEA domain family member 2/ETF antibody (ab92279) at 1 µg/ml + Human ileum lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (4)
ab92279 被引用在 4 文献中.
- Holmes B et al. mTORC2-mediated direct phosphorylation regulates YAP activity promoting glioblastoma growth and invasive characteristics. Neoplasia 23:951-965 (2021). PubMed: 34343821
- Chang Y et al. PHF5A promotes colorectal cancerprogression by alternative splicing of TEAD2. Mol Ther Nucleic Acids 26:1215-1227 (2021). PubMed: 34853721
- Liu G et al. Hsa_circ_0085576 promotes clear cell renal cell carcinoma tumorigenesis and metastasis through the miR-498/YAP1 axis. Aging (Albany NY) 12:11530-11549 (2020). PubMed: 32541093
- Wang J et al. Effect of TEAD4 on multilineage differentiation of muscle-derived stem cells. Am J Transl Res 10:998-1011 (2018). WB ; Cow . PubMed: 29636889