概述

  • 产品名称Anti-TDRD7抗体
    参阅全部 TDRD7 一抗
  • 描述
    兔多克隆抗体to TDRD7
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 807-835 of Human TDRD7 (NP_055105.2).

  • 阳性对照
    • A549 cell lysate; Mouse bladder tissue lysate; Human brain tissue. This antibody gave a positive result when used in the following methanol fixed cell lines: CACO-2.

性能

应用

Our Abpromise guarantee covers the use of ab107675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 124 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 序列相似性Contains 2 Tudor domains.
  • 细胞定位Cytoplasm. Present in chromatoid body (CB) of spermatids (mammalian counterpart of germplasm, pole plasm or polar granules in Drosophila germ cells), also named processing bodies (P-bodies) in somatic cells. Detected in the multilobular cytoplasmic CBs (also called intermitochondrial cementin) in pachytene spermatocytes and as a single perinuclear CB in haploid round spermatids.
  • Information by UniProt
  • 数据库链接
  • 形式Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. Found in a complex containing CABLES1, CDK16 and CDK17. Interacts with CABLES1, CDK17 and PIWIL1.
  • 别名
    • CATC4 antibody
    • KIAA1529 antibody
    • PCTAIRE 2 antibody
    • PCTAIRE2 binding protein antibody
    • PCTAIRE2 BP antibody
    • PCTAIRE2-binding protein antibody
    • PCTAIRE2BP antibody
    • RP11-508D10.1 antibody
    • TDRD 7 antibody
    • Tdrd7 antibody
    • TDRD7_HUMAN antibody
    • TrAP antibody
    • Tudor domain containing 7 antibody
    • Tudor domain-containing protein 7 antibody
    • Tudor repeat associator with PCTAIRE 2 antibody
    see all

Anti-TDRD7 antibody 图像

  • ICC/IF image of ab107675 stained CACO-2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107675 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-TDRD7 antibody (ab107675) at 1/100 dilution + A549 cell lysate at 35 µg

    Predicted band size : 124 kDa
  • Anti-TDRD7 antibody (ab107675) at 1/100 dilution + Mouse bladder tissue lysate at 35 µg

    Predicted band size : 124 kDa
  • ab107675, at 1/50 dilution, staining TDRD7 in formalin-fixed, paraffin-embedded Human brain tissue by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.

Anti-TDRD7 antibody (ab107675)参考文献

ab107675 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab107675.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"