Anti-TDRD7抗体(ab103866)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to TDRD7
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Cow, Dog, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human TDRD7.

  • 阳性对照
    • This antibody gave a positive signal in both Human testis and lung tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab103866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 124 kDa (predicted molecular weight: 124 kDa).

靶标

  • 序列相似性
    Contains 2 Tudor domains.
  • 细胞定位
    Cytoplasm. Present in chromatoid body (CB) of spermatids (mammalian counterpart of germplasm, pole plasm or polar granules in Drosophila germ cells), also named processing bodies (P-bodies) in somatic cells. Detected in the multilobular cytoplasmic CBs (also called intermitochondrial cementin) in pachytene spermatocytes and as a single perinuclear CB in haploid round spermatids.
  • Information by UniProt
  • 数据库链接
  • 形式
    Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. Found in a complex containing CABLES1, CDK16 and CDK17. Interacts with CABLES1, CDK17 and PIWIL1.
  • 别名
    • CATC4 antibody
    • KIAA1529 antibody
    • PCTAIRE 2 antibody
    • PCTAIRE2 binding protein antibody
    • PCTAIRE2 BP antibody
    • PCTAIRE2-binding protein antibody
    • PCTAIRE2BP antibody
    • RP11-508D10.1 antibody
    • TDRD 7 antibody
    • Tdrd7 antibody
    • TDRD7_HUMAN antibody
    • TrAP antibody
    • Tudor domain containing 7 antibody
    • Tudor domain-containing protein 7 antibody
    • Tudor repeat associator with PCTAIRE 2 antibody
    see all

图片

  • All lanes : Anti-TDRD7 antibody (ab103866) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 124 kDa
    Observed band size : 124 kDa
    Additional bands at : 37 kDa,89 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

实验方案

文献

ab103866 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Bos taurus Tissue lysate - whole (ovary, testis and oocytes)
Gel Running Conditions
Reduced Denaturing (gel 8%)
Loading amount
25 µg
Specification
ovary, testis and oocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Verified customer

提交于 Feb 09 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (gel 8%)
Sample
Human Purified protein (Periodontal ligament cell-conditioned medium)
Specification
Periodontal ligament cell-conditioned medium
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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提交于 Oct 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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