概述

  • 产品名称Anti-TDP43抗体[3H8]
    参阅全部 TDP43 一抗
  • 描述
    小鼠单克隆抗体[3H8] to TDP43
  • 经测试应用适用于: WB, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: a wide range of other species
  • 免疫原

    Recombinant full length Human His-tagged TARDBP, expressed in E.coli

  • 阳性对照
    • Mouse brain tissue lysate, rat brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab104223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Predicted molecular weight: 45 kDa.
ICC/IF 1/1000.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.
  • 组织特异性Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.
  • 疾病相关Defects in TARDBP are the cause of amyotrophic lateral sclerosis type 10 (ALS10) [MIM:612069]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of ALS is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.
  • 序列相似性Contains 2 RRM (RNA recognition motif) domains.
  • 结构域The RRM domains can bind to both DNA and RNA.
  • 翻译后修饰Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
    Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
    Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
  • 细胞定位Nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALS10 antibody
    • OTTHUMP00000002171 antibody
    • OTTHUMP00000002172 antibody
    • OTTHUMP00000002173 antibody
    • TADBP_HUMAN antibody
    • TAR DNA binding protein 43 antibody
    • TAR DNA binding protein antibody
    • TAR DNA-binding protein 43 antibody
    • TARDBP antibody
    • TDP 43 antibody
    • TDP-43 antibody
    • TDP43 antibody
    see all

Anti-TDP43 antibody [3H8] 图像

  • ab104223 at 1/1000 dilution, staining TARDBP in rat brain tissue (red). Chicken antibody to GFAP CPCA-GFAP (green) shows the processes of astrocytic glial cells. Nuclei of all cells are revealed with DAPI DNA stain (blue).


  • Predicted band size : 45 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: TDP43 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab104223 observed at 48 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab104223 was shown to specifically react with TDP43 when TDP43 knockout samples were used. Wild-type and TDP43 knockout samples were subjected to SDS-PAGE.  Ab104223 and ab181602 (loading control to GAPDH) were diluted at 1/5000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) and IRDye® 680 Goat anti-Rabbit IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Overlay histogram showing JEG3 cells stained with ab104223 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab104223, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in JEG3 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-TDP43 antibody [3H8] (ab104223)参考文献

This product has been referenced in:
  • Fang YS  et al. Full-length TDP-43 forms toxic amyloid oligomers that are present in frontotemporal lobar dementia-TDP patients. Nat Commun 5:4824 (2014). Mouse . Read more (PubMed: 25215604) »

See 1 Publication for this product

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I can confirm that ab104223 Anti-TARDBP antibody [3H8] sold as ascites.

Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet.

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