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Our Abpromise guarantee covers the use of ab57105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|WB||Use a concentration of 1 - 5 µg/ml.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml.|
|Sandwich ELISA||Use at an assay dependent concentration.|
TDP43 antibody (ab57105) at 1ug/lane + A-431 cell lysate at 25ug/lane.
ab57105 staining TDP43 in HeLa cells. Antibody concentration 10µg/ml.
ab57105 staining TDP43 in human leiomyosarcoma tissue. Antibody concentration 3µg/ml.
Detection limit for recombinant GST tagged TDP43 is 0.3 ng/ml as a capture antibody.
ab57105 staining TDP43 overexpressed in the HEK293 cell line (cotransfected with TDP43 validated Chimera siRNA) (lane 1) or non-tranfected control (lane 2).
GAPDH used as a specificity and loading control.