Anti-TDP1抗体[AT1F2] (ab91035)

概述

  • 产品名称
    Anti-TDP1抗体[AT1F2]
    参阅全部 TDP1 一抗
  • 描述
    小鼠单克隆抗体[AT1F2] to TDP1
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to the N terminal amino acids 1-298 of Human TDP1, purified from E. coli.

  • 阳性对照
    • MCF7 cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    AT1F2
  • 同种型
    IgG2b
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab91035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Predicted molecular weight: 68 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能
    DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate.
  • 组织特异性
    Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus.
  • 疾病相关
    Spinocerebellar ataxia, autosomal recessive, with axonal neuropathy
  • 序列相似性
    Belongs to the tyrosyl-DNA phosphodiesterase family.
  • 翻译后修饰
    Phosphorylated on serine and/or threonine residues, but not on tyrosine residues.
  • 细胞定位
    Nucleus. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI838772 antibody
    • AW493413 antibody
    • FLJ11090 antibody
    • MGC104252 antibody
    • MGC112732 antibody
    • RP24-311F12.2 antibody
    • SCAN1 antibody
    • TDP1 antibody
    • TYDP antibody
    • TYDP1_HUMAN antibody
    • Tyr-DNA phosphodiesterase 1 antibody
    • Tyrosyl-DNA phosphodiesterase 1 antibody
    see all

图片

  • Anti-TDP1 antibody [AT1F2] (ab91035) at 1/1000 dilution + MCF7 cell lysate at 30 µg

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 68 kDa

实验方案

文献

ab91035 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab91035.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册