概述

  • 产品名称
  • 描述
    兔多克隆抗体to TDO2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Pig
  • 免疫原

    Synthetic peptide corresponding to N terminal amino acids 2-51 (SGCPFLGNNF GYTFKKLPVE GSEEDKSQTG VNRASKGGLI YGNYLHLEKV) of human TDO2 (NP_005642).

  • 阳性对照
    • Human fetal heart lysate

性能

应用

Our Abpromise guarantee covers the use of ab84926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Incorporates oxygen into the indole moiety of tryptophan. Has a broad specificity towards tryptamine and derivatives including D- and L-tryptophan, 5-hydroxytryptophan and serotonin.
  • 通路
    Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
  • 序列相似性
    Belongs to the tryptophan 2,3-dioxygenase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3-dioxygenase antibody
    • T23O_HUMAN antibody
    • TDO 2 antibody
    • TDO antibody
    • tdo2 antibody
    • TO antibody
    • TPH2 antibody
    • TRPO antibody
    • Tryptamin 2 3 dioxygenase antibody
    • Tryptamin 2 antibody
    • Tryptophan 2 3 dioxygenase antibody
    • Tryptophan 2 antibody
    • Tryptophan oxygenase antibody
    • Tryptophan pyrrolase antibody
    • Tryptophanase antibody
    see all

图片

  • Anti-TDO2 antibody (ab84926) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab84926 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Mar 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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