Anti-TCR V beta 5抗体[MEM-262] (ab1898)

概述

  • 产品名称
    Anti-TCR V beta 5抗体[MEM-262]
  • 描述
    小鼠单克隆抗体[MEM-262] to TCR V beta 5
  • 特异性
    The antibody recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
  • 经测试应用
    适用于: Flow Cyt, IP, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human thymoma cell line HPB-ALL.

  • 常规说明
    Functional Application Activates T cells (V beta 5-related subset).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    >95% by SDS-PAGE
  • 纯化说明
    Purified from hybridoma culture supernatant by protein A affinity chromatography.
  • 克隆
    单克隆
  • 克隆编号
    MEM-262
  • 同种型
    IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab1898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
IP
WB
  • 应用说明
    Flow Cyt: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Uniquely recognizes denatured TCR beta chains under the conditions of Western blotting.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. The Vß5 TCR is a variant of the TCR ß chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR Vß5 has been reported to recognize some staphylococcus bacterial superantigens.
    • 细胞定位
      Cell Membrane
    • 数据库链接
    • 别名
      • T cell receptor antibody
      • TCB antibody
      • TCRB antibody
      • TRB antibody
      • TRB@ antibody
      see all

    文献

    This product has been referenced in:
    • Wang CT  et al. Attenuating systemic inflammatory markers in simulated high-altitude exposure by heat shock protein 70-mediated hypobaric hypoxia preconditioning in rats. J Formos Med Assoc 114:328-38 (2015). Read more (PubMed: 25839766) »
    • Grabherr MG  et al. Exploiting nucleotide composition to engineer promoters. PLoS One 6:e20136 (2011). Read more (PubMed: 21625601) »

    See all 3 Publications for this product

    客户评价及客户问答

    Thank you for your patience. For ab9219, the originator has recommended the following. Since the antibody recognizes human cytokeratin 18 we recommend using the PBS-insoluble fraction (pellet) of human cell lines containing cytokeratin 18 (for example...

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    Thank you for your enquiry. The originator of ab1898 was able to provide me with the following information. Western blot detection: -material: HPB-ALL cell line -non-reducing conditions -detects only free beta chains -molecular weight 36 kDa ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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