概述

  • 产品名称Anti-TCR V beta 10抗体[G101]
  • 描述
    小鼠单克隆抗体[G101] to TCR V beta 10
  • 特异性Approximately 4.2% of unseparated LEW lymph node cells were detected by this clone. Reactivity is restricted to alpha/beta T cells, of which 6.7% labelled.
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Tissue/ cell preparation (Rat): T cell clone expressing the TCR V beta 10 gene product

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明ab23348 has been purified by ion exchange chromatography from tissue culture supernatant.
  • 克隆单克隆
  • 克隆编号G101
  • 骨髓瘤P3x63-Ag8.653
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab23348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明Flow Cyt: Use 10µl for 106 cells in 100µl, or 1/50 - 1/100.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Several V segments and one J segment of the beta locus are known to be incapable of encoding a protein and are considered pseudogenes. The beta locus also includes eight trypsinogen genes, three of which encode functional proteins and five of which are pseudogenes. Chromosomal abnormalities involving the T-cell receptor beta locus have been associated with T-cell lymphomas.
    • 细胞定位Plasma membrane
    • 数据库链接

    Anti-TCR V beta 10 antibody [G101] (ab23348)参考文献

    This product has been referenced in:
    • Torres-Nagel NE  et al. Identification of rat Tcrb-V8.2, 8.5, and 10 gene products by monoclonal antibodies. Immunogenetics 37:305-8 (1993). Read more (PubMed: 8420840) »

    See 1 Publication for this product

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