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The details of the immunogen for this antibody are not available.
ab25678 reacts with approximately 40% of thymocytes, 40-50% of blood mononuclear cells and 40% of splenocytes in the chicken. Two-color immunofluorescence has revealed that most of the TCR2+ thymocytes express both CD4 and CD8 antigens. The TCR2+ cells in blood were found to express either CD4 (74±2%) or CD8 (26±4%). TCR2+ cells in the spleen also express either CD4 (37±1%) or CD8 (64±4%). Surprisingly, a relatively large subpopulation of CD8 + cells in the spleen are negative for TCR2. This observation led to the demonstration that 71±6% of the TCR1+ cells in the spleen express the CD8 marker.
Our Abpromise guarantee covers the use of ab25678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.
In ovo suppression of the development of TCR-Vbeta1 cells.
|Flow Cyt||Use 1µg for 106 cells. ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
Chicken PBMC were double stained with ab25678 (1µg/106 cells) and mouse anti-chicken CD3-R-PE. Small lymphocytes were gated and subjected to 2-color analysis on a FACScan.