The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.125-0.25µg for 105 cells. Cell number should be determined empirically but can range from 105 to 108 cells/test (based on a test volume of 100 µl).
Ab157805-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
ab157805-Armenian Hamster polyclonal IgG, is suitable for use as an isotype control with this antibody.
相关性The receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain.
T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.