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TCR affinity purified from T-cell hybridoma DO-11.10 (Mouse)
ab25010 can be used for in vitro stimulation of alpha beta TCR-expressing T cells and in vitro and in vivo depletion of aß TCR-bearing cells. Plate-bound or soluble ab25010 activates aß TCR bearing T cells.
Our Abpromise guarantee covers the use of ab25010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 2µg for 106 cells.|
|IHC-Fr||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
ab25010, Hamster monoclonal [H57-597] to TCR alpha + TCR beta (FITC), staining mouse (BALB/c) splenocytes by flow cytometry. 1µg antibody was used / 106 cells. The cells were double stained with a PE-conjugated rat anti-mouse CD19 and then analysed on a FACScan flow cytometer.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"