Anti-TCR alpha + TCR beta抗体[H57-597] (ab25336)

概述

  • 产品名称Anti-TCR alpha + TCR beta抗体[H57-597]
    参阅全部 TCR alpha + TCR beta 一抗
  • 描述
    亚美尼亚仓鼠单克隆抗体[H57-597] to TCR alpha + TCR beta
  • 特异性ab25336 reacts with a common epitope of the ß chain of the mouse aß T cell receptor (TCR) complex.
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IP, Othermore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    TCR affinity purified from T-cell hybridoma DO-11.10 (Mouse)

  • 常规说明


    ab25336 does not react with yd TCR-bearing cells. Plate-bound or soluble ab25336 activates aß TCR-bearing T cells. In vitro and in vivo, ab25336 can induce immature thymocytes to undergo apoptosis. In vivo administration of ab25336 has been shown to deplete aß TCR-bearing cells to near completion and prevent graft rejection.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • 纯度IgG fraction
  • Primary antibody说明ab25336 does not react with yd TCR-bearing cells. Plate-bound or soluble ab25336 activates aß TCR-bearing T cells. In vitro and in vivo, ab25336 can induce immature thymocytes to undergo apoptosis. In vivo administration of ab25336 has been shown to deplete aß TCR-bearing cells to near completion and prevent graft rejection.
  • 克隆单克隆
  • 克隆编号H57-597
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab25336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. ab18479-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Other Use at an assay dependent concentration. In vitro stimulation of ab TCR-expressing T cells and In vitro and in vivo depletion of ab TCR-bearing cells.

靶标

  • 相关性The receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.
  • 细胞定位Type I membrane protein.
  • 数据库链接
  • 别名
    • FLJ22602 antibody
    • MGC117436 antibody
    • MGC22624 antibody
    • MGC23964 antibody
    • MGC71411 antibody
    • T cell antigen receptor alpha polypeptide antibody
    • T cell antigen receptor beta polypeptide T cell receptor beta cluster antibody
    • T cell receptor alpha (V D J C) antibody
    • T cell receptor alpha chain C region antibody
    • T cell receptor alpha constant antibody
    • T cell receptor alpha locus antibody
    • T cell receptor beta cluster antibody
    • T cell receptor beta locus antibody
    • TCRA antibody
    • TCRB antibody
    • TCRD antibody
    • TRA antibody
    • TRAC antibody
    • TRB antibody
    see all

Anti-TCR alpha + TCR beta antibody [H57-597] (ab25336)参考文献

This product has been referenced in:
  • Shi YF  et al. In vivo administration of monoclonal antibodies to the CD3 T cell receptor complex induces cell death (apoptosis) in immature thymocytes. J Immunol 146:3340-6 (1991). Read more (PubMed: 1827483) »
  • Murosaki S  et al. Failure of T cell receptor V beta negative selection in murine intestinal intra-epithelial lymphocytes. Int Immunol 3:1005-13 (1991). Read more (PubMed: 1661605) »

See all 8 Publications for this product

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Thank you for contacting us yesterday in regards to the anti-TCR alpha + TCR beta antibody [H57-597] (ab25336).

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Thank you for taking the time to fill in our technical questionaire and send it in to us. I am sorry you have had difficulty obtaining satisfactory results in immunohistochemistry with this antibody. I have read through the details and would like to ma...

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