Anti-TCR alpha抗体(ab18861)

概述

  • 产品名称Anti-TCR alpha抗体
    参阅全部 TCR alpha 一抗
  • 描述
    兔多克隆抗体to TCR alpha
  • 经测试应用适用于: IHC-P, WB, IP, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein (Human)

性能

应用

Our Abpromise guarantee covers the use of ab18861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
WB
IP
IHC-Fr
  • 应用说明IHC-Fr: Use at a concentration of 15 - 25 µg/ml.
    IP: Use at a concentration of 10 - 20 µg/ml.
    WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 16 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性TCR (T cell antigen receptor) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either alpha and beta or gamma and delta chains. CD3 chains and the CD4 or CD8 coreceptors are also required for efficient signal transduction through the TCR.
    • 细胞定位Plasma membrane
    • 数据库链接
    • 别名
      • T cell antigen receptor alpha polypeptide antibody
      • T cell receptor alpha antibody
      • T cell receptor alpha locus antibody
      • T-cell receptor alpha chain C region antibody
      • TCRA antibody
      • TCRD antibody
      • TRA antibody
      • TRA@ antibody
      • TRAC antibody
      see all

    Anti-TCR alpha antibody 图像

    • All lanes : Anti-TCR alpha antibody (ab18861) at 1 µg/ml

      Lane 1 : Human thymus tissue lysate - total protein (ab30146)
      Lane 2 : Human spleen tissue lysate - total protein (ab29699)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 16 kDa
      Observed band size : 21 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 56 kDa,74 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 90 seconds

    Anti-TCR alpha antibody (ab18861)参考文献

    ab18861 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Tonsil)
    Specification Tonsil
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization No
    Blocking step BSA in FCS (20ml) + DMEM (80ml) as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
    Username

    Dr. James Jupp

    Verified customer

    提交于 Nov 06 2009

    Thank you for your enquiry. To our knowledge, the antibody has yet to be tested in these applications, and I am sorry this means I am unable to supply you with further details on FACS or its functionality as a stimulating antibody. All tested appli...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application ELISA
    Sample Not applicable Recombinant protein (Human/mouse chimeric alpha/beta TCR)
    Specification Human/mouse chimeric alpha/beta TCR
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2%
    Type Sandwich
    Username

    Abcam user community

    Verified customer

    提交于 Apr 19 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"