Anti-TCR alpha + TCR beta抗体[H57-597] (ab25336)
Key features and details
- Armenian hamster monoclonal [H57-597] to TCR alpha + TCR beta
- Suitable for: IHC-Fr, IP, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-TCR alpha + TCR beta抗体[H57-597]
参阅全部 TCR alpha + TCR beta 一抗 -
描述
亚美尼亚仓鼠单克隆抗体[H57-597] to TCR alpha + TCR beta -
宿主
Armenian hamster -
特异性
ab25336 reacts with a common epitope of the ß chain of the mouse aß T cell receptor (TCR) complex. -
经测试应用
适用于: IHC-Fr, IP, Flow Cytmore details -
种属反应性
与反应: Mouse -
免疫原
TCR affinity purified from T-cell hybridoma DO-11.10 (Mouse)
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常规说明
ab25336 does not react with yd TCR-bearing cells. Plate-bound or soluble ab25336 activates aß TCR-bearing T cells. In vitro and in vivo, ab25336 can induce immature thymocytes to undergo apoptosis. In vivo administration of ab25336 has been shown to deplete aß TCR-bearing cells to near completion and prevent graft rejection.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.14% Monobasic dihydrogen sodium phosphate -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
Purified from tissue culture supernatant -
Primary antibody说明
ab25336 does not react with yd TCR-bearing cells. Plate-bound or soluble ab25336 activates aß TCR-bearing T cells. In vitro and in vivo, ab25336 can induce immature thymocytes to undergo apoptosis. In vivo administration of ab25336 has been shown to deplete aß TCR-bearing cells to near completion and prevent graft rejection. -
克隆
单克隆 -
克隆编号
H57-597 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25336于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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IF |
Use at an assay dependent concentration.
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说明 |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
IF
Use at an assay dependent concentration. |
靶标
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相关性
The receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. -
细胞定位
Type I membrane protein. -
数据库链接
- Entrez Gene: 21473 Mouse
- Entrez Gene: 21577 Mouse
- SwissProt: P01849 Mouse
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别名
- FLJ22602 antibody
- MGC117436 antibody
- MGC22624 antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab25336 被引用在 10 文献中.
- Gramlich OW et al. T and B Lymphocyte Deficiency in Rag1-/- Mice Reduces Retinal Ganglion Cell Loss in Experimental Glaucoma. Invest Ophthalmol Vis Sci 61:18 (2020). PubMed: 33320171
- Liang W et al. Core Fucosylation of the T Cell Receptor Is Required for T Cell Activation. Front Immunol 9:78 (2018). PubMed: 29434598
- Shi YF et al. In vivo administration of monoclonal antibodies to the CD3 T cell receptor complex induces cell death (apoptosis) in immature thymocytes. J Immunol 146:3340-6 (1991). PubMed: 1827483
- Murosaki S et al. Failure of T cell receptor V beta negative selection in murine intestinal intra-epithelial lymphocytes. Int Immunol 3:1005-13 (1991). PubMed: 1661605
- Finkel TH et al. Alpha beta T cell receptor and CD3 transduce different signals in immature T cells. Implications for selection and tolerance. J Immunol 142:3006-12 (1989). PubMed: 2523422
- Crago SS et al. Antisera to the secretory component recognize the murine Fc receptor for IgA. J Immunol 142:3909-12 (1989). PubMed: 2785569
- Zhang JW et al. A common epitope on human myelin basic protein and the human T lymphocyte CD3 molecule. J Immunol 142:3917-22 (1989). PubMed: 2469727
- Byrne JA et al. Virgin and memory T cells have different requirements for activation via the CD2 molecule. Int Immunol 1:29-35 (1989). PubMed: 2577287
- Kubo RT et al. Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. J Immunol 142:2736-42 (1989). PubMed: 2467936
- Finkel TH et al. The thymus has two functionally distinct populations of immature alpha beta + T cells: one population is deleted by ligation of alpha beta TCR. Cell 58:1047-54 (1989). PubMed: 2673539