Anti-TCP11L2抗体(ab87197)

概述

  • 产品名称
    Anti-TCP11L2抗体
    参阅全部 TCP11L2 一抗
  • 描述
    兔多克隆抗体to TCP11L2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 108-157 (HQAFWDVLDS ELNADPPEFE HAIKLFEEIR EILLSFLTPG GNRLRNQICE) of Human TCP11L2 (NP_689985).

  • 阳性对照
    • Human fetal heart lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab87197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-TCP11L2 antibody (ab87197) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Additional bands at : <22 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab87197 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Application
Western blot
Sample
Human Cell lysate - other (Haematopoietic Cell Line)
Loading amount
30 µg
Specification
Haematopoietic Cell Line
Treatment
1uM PI-103 +- L-685 1uM for 72hr
Gel Running Conditions
Non-reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Clare Shepherd

Verified customer

提交于 Jul 31 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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