Recombinant fragment corresponding to amino acids 2-140 of Human TCP11L1.
- Human lymph node tissue; Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in HEK293T cells
Shipped at 4°C. Store at -20ºC.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/250 - 1/500.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-TCP11L1 antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-TCP11L1 antibody (ab121231)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121231 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-TCP11L1 antibody (ab121231)
All lanes : Anti-TCP11L1 antibody (ab121231) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in HEK293T cells
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP11L1 antibody (ab121231)
ab121231, at 1/100 dilution, staining TCP11L1 in Human lymph node tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
Note: Shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.
Anti-TCP11L1 antibody (ab121231)参考文献
has not yet been referenced specifically in any publications.
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