概述

  • 产品名称Anti-TCP11L1抗体
    参阅全部 TCP11L1 一抗
  • 描述
    兔多克隆抗体to TCP11L1
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 2-140 of Human TCP11L1.

  • 阳性对照
    • Human lymph node tissue; Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in HEK293T cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20ºC.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-TCP11L1 antibody 图像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121231 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-TCP11L1 antibody (ab121231) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in HEK293T cells
  • ab121231, at 1/100 dilution, staining TCP11L1 in Human lymph node tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

    Note: Shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.

Anti-TCP11L1 antibody (ab121231)参考文献

ab121231 has not yet been referenced specifically in any publications.

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