概述
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产品名称Anti-TCP1 theta抗体
参阅全部 TCP1 theta 一抗 -
描述兔多克隆抗体to TCP1 theta
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宿主Rabbit
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经测试应用适用于: ICC/IF, WB, IHC-Pmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Cow, Zebrafish
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免疫原
Recombinant fragment, corresponding to a region within internal sequence amino acids 111 - 337 of Human TCP1 theta.
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阳性对照
- 293T, A431, H1299, HeLa, HepG2, Molt-4 or Raji cell lysate. IF/ICC: HeLa cell line.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 1% BSA, 1X PBS, pH 7.0 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab96321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 60 kDa. | |
| IHC-P | 1/100 - 1/500. |
靶标
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相关性TCP1 theta is the theta polypeptide of the CCT chaperonin molecule complex. The intact CCT complex is composed of eight polypeptides in a double ring. TCP1 theta is a molecular chaperone that assists the folding of proteins upon ATP hydrolysis. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.
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细胞定位Cytoplasm. Cytoplasm; cytoskeleton; centrosome
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数据库链接
- Entrez Gene: 281047 Cow
- Entrez Gene: 10694 Human
- Entrez Gene: 12469 Mouse
- Entrez Gene: 288305 Rat
- SwissProt: Q3ZCI9 Cow
- SwissProt: P50990 Human
- SwissProt: P42932 Mouse
- Unigene: 125113 Human
see all -
别名
- C21orf112 antibody
- CCT 8 antibody
- CCT theta antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-TCP1 theta antibody (ab96321)ICC/IF image of ab96321 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96321, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-TCP1 theta antibody (ab96321)All lanes : Anti-TCP1 theta antibody (ab96321) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 60 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 theta antibody (ab96321)Immunohistochemistry analysis of paraffin-embedded OVCAR3 xenograft, using ab96321 at 1/500 dilution.
实验方案
文献
ab96321 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"