Anti-TCP1 theta抗体(ab96321)

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概述

  • 产品名称
    Anti-TCP1 theta抗体
    参阅全部 TCP1 theta 一抗
  • 描述
    兔多克隆抗体to TCP1 theta
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Zebrafish
  • 免疫原

    Recombinant fragment, corresponding to a region within internal sequence amino acids 111 - 337 of Human TCP1 theta.

  • 阳性对照
    • 293T, A431, H1299, HeLa, HepG2, Molt-4 or Raji cell lysate. IF/ICC: HeLa cell line.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 1% BSA, 1X PBS, pH 7.0
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/500.

靶标

  • 相关性
    TCP1 theta is the theta polypeptide of the CCT chaperonin molecule complex. The intact CCT complex is composed of eight polypeptides in a double ring. TCP1 theta is a molecular chaperone that assists the folding of proteins upon ATP hydrolysis. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.
  • 细胞定位
    Cytoplasm. Cytoplasm; cytoskeleton; centrosome
  • 数据库链接
    see all
  • 别名
    • C21orf112 antibody
    • CCT 8 antibody
    • CCT theta antibody
    • CCT8 antibody
    • CCTQ antibody
    • Chaperonin containing TCP1 subunit 8 (theta) antibody
    • Chaperonin subunit 8 (Theta) (Predicted), isoform CRA_a antibody
    • Chaperonin subunit 8 (theta) antibody
    • Chromosome 21 open reading frame 112 antibody
    • D21S246 antibody
    • KIAA0002 antibody
    • PRED71 antibody
    • Renal carcinoma antigen NY REN 15 antibody
    • T complex protein 1 subunit theta antibody
    • T complex protein 1 theta subunit antibody
    • TCP 1 theta antibody
    • Tcpq antibody
    see all

Anti-TCP1 theta antibody 图像

  • Immunocytochemistry/ Immunofluorescence - Anti-TCP1 theta antibody (ab96321)
    Immunocytochemistry/ Immunofluorescence - Anti-TCP1 theta antibody (ab96321)

    ICC/IF image of ab96321 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96321, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - TCP1 theta antibody (ab96321)
    Western blot - TCP1 theta antibody (ab96321)
    All lanes : Anti-TCP1 theta antibody (ab96321) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 60 kDa
    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCP1 theta antibody (ab96321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCP1 theta antibody (ab96321)
    Immunohistochemistry analysis of paraffin-embedded OVCAR3 xenograft, using ab96321 at 1/500 dilution.

Anti-TCP1 theta antibody (ab96321)参考文献

ab96321 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab96321？请让我们知道，以便我们可以引用本数据表中的参考文章。

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Votes: Highest First

In our experiments we found something new about T-complex protein 1 subunit theta.We want to check our experiments with WB. Because of that I wanted to order sample of antibody.I sent an e-mail to abcam but they said "We do not offer free or trial...

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Thank you for contacting us with your question.

The TCP1 theta antibody ab96321 has already been tested in Western blot with human lysates, so we will fully guarantee it to work in this species and application. We do not have small samples of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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