Anti-TCP1 epsilon抗体(ab82778)


  • 产品名称Anti-TCP1 epsilon抗体
    参阅全部 TCP1 epsilon 一抗
  • 描述
    兔多克隆抗体to TCP1 epsilon
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 492-541 (DCLHKGTNDM KQQHVIETLI GKKQQISLAT QMVRMILKID DIRKPGESEE) of human TCP1 epsilon (NP_036205).

  • 阳性对照
    • 293T cell lysate.



Our Abpromise guarantee covers the use of ab82778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 5 µg/ml.


  • 相关性TCP1 epsilon is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP dependent manner. The complex folds various proteins, including actin and tubulin.
  • 细胞定位Cytoplasm; cytoskeleton and centrosome.
  • 数据库链接
  • 别名
    • CCT 5 antibody
    • CCT epsilon antibody
    • CCT5 antibody
    • CCTE antibody
    • CCTepsilon antibody
    • Chaperonin containing T-complex polypeptide 1, subunit 5 antibody
    • Chaperonin containing TCP 1 epsilon antibody
    • Chaperonin containing TCP 1 subunit 5 antibody
    • Chaperonin containing TCP 1 subunit 5 epsilon antibody
    • Chaperonin containing TCP1 epsilon antibody
    • Chaperonin containing TCP1 subunit 5 antibody
    • Chaperonin containing TCP1 subunit 5 epsilon antibody
    • KIAA0098 antibody
    • T complex protein 1 epsilon subunit antibody
    • T complex protein 1 subunit epsilon antibody
    • TCP 1 epsilon antibody
    • TCP1epsilon antibody
    • TCPE antibody
    see all

Anti-TCP1 epsilon antibody 图像

  • Anti-TCP1 epsilon antibody (ab82778) at 1 µg/ml ( 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 20 kDa,24 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab82778 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82778, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TCP1 epsilon antibody (ab82778)参考文献

ab82778 has not yet been referenced specifically in any publications.

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