概述

  • 产品名称Anti-TCP1 delta抗体
    参阅全部 TCP1 delta 一抗
  • 描述
    兔多克隆抗体to TCP1 delta
  • 经测试应用适用于: WB, ELISA, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide: AGLNPISTVT ELRNRHAQGE KTAGINVRKG GISNILEELV VQPLLVSVSA, corresponding to a region within C terminal amino acids 469-518 of Human TCP1 delta

  • 阳性对照
    • HepG2 cell lysates

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab49151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.
ICC/IF 1/200.
IHC-P 1/100.

靶标

  • 相关性The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back to back rings, each containing 8 unique but homologous subunits (e.g. CCT3). CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha transducin. TCP1 delta belongs to the TCP1 chaperonin family, and is a molecular chaperone; assisting the folding of proteins upon ATP hydrolysis. It is known to play a role, in vitro, in the folding of actin and tubulin.
  • 细胞定位Cytoplasmic > Cytoskeleton
  • 数据库链接
  • 别名
    • CCT delta antibody
    • CCT4 antibody
    • CCTD antibody
    • chaperonin containing t-complex polypeptide 1, delta subunit antibody
    • chaperonin containing TCP1, subunit 4 (delta) antibody
    • SRB antibody
    • Stimulator of TAR RNA binding antibody
    • T complex protein 1 subunit delta antibody
    • TCP-1-delta antibody
    see all

Anti-TCP1 delta antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling TCP1 delta with ab49151 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - TCP1. Right - Merge.


  • Predicted band size : 60 kDa
    Western blot analysis of HepG2 cell lysate labeling TCP1 delta with ab49151 at 1µg/ml.
  • Immunocytochemistry/immunofluorescence analysis of Rat hippocampal neurons - 14DIV labeling TCP1 delta with ab49151 at 1/200. A Cy3-conjugated anti rabbit IgG (1/500) was used as the secondary antibody.


  • Predicted band size : 60 kDa
    Western blot analysis of HepG2 cell lysate labeling TCP1 delta with ab49151 at 1:1000. A Cy3-conjugated anti-rabbit IgG (1:10000) was used as the secondary antibody.
  • All lanes : Anti-TCP1 delta antibody (ab49151) at 1.25 µg/ml (in 5% skim milk / PBS buffer)

    Lane 1 : molecular marker
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 56 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%

Anti-TCP1 delta antibody (ab49151)参考文献

This product has been referenced in:
  • Mallawaaratchy DM  et al. The phosphoinositide 3-kinase inhibitor LY294002, decreases aminoacyl-tRNA synthetases, chaperones and glycolytic enzymes in human HT-29 colorectal cancer cells. J Proteomics 75:1590-9 (2012). WB ; Human . Read more (PubMed: 22172953) »

See 1 Publication for this product

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