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RabMAb

Anti-TCP1 alpha抗体[EPR4082] (ab109126)

概述

  • 产品名称
    Anti-TCP1 alpha抗体[EPR4082]
    参阅全部 TCP1 alpha 一抗
  • 描述
    兔单克隆抗体[EPR4082] to TCP1 alpha
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, WB, IP, IHC-Pmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human
  • 免疫原

    Synthetic peptide within Human TCP1 alpha aa 500-600. The exact sequence is proprietary.

  • 阳性对照
    • HeLa, ECV-304, MCF7 and Daudi cell lysates; Human testis tissue
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab109126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250.
  • 应用说明
    Is unsuitable for Flow Cyt.
  • 靶标

    • 功能
      Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
    • 序列相似性
      Belongs to the TCP-1 chaperonin family.
    • 细胞定位
      Cytoplasm. Cytoplasm > cytoskeleton > centrosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AI528772 antibody
      • c-cpn antibody
      • CCT alpha antibody
      • CCT antibody
      • CCT-alpha antibody
      • CCT1 antibody
      • Ccta antibody
      • CCTalpha antibody
      • D6S230E antibody
      • MGC133746 antibody
      • p63 antibody
      • T complex 1 antibody
      • T complex protein 1 alpha subunit antibody
      • T complex protein 1 antibody
      • T-complex homolog TCP1 antibody
      • T-complex protein 1 subunit alpha antibody
      • T-complex protein 1 subunit alpha B antibody
      • Tailless complex polypeptide 1 antibody
      • Tailless complex polypeptide 1A antibody
      • Tailless complex polypeptide 1B antibody
      • TCP 1 alpha antibody
      • Tcp-1 antibody
      • TCP-1-alpha antibody
      • TCP1 antibody
      • TCPA_HUMAN antibody
      • Tp63 antibody
      • TRic antibody
      see all

    图片

    • ab109126 at 1:50 dilution (5μg in 1mg lysate) immunoprecipitating TCP1 alpha in ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate. 

       

      Lane 1: ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate

      Lane 2: PBS instead of ab109126 in ECV-304 whole cell lysate


      For western blotting, Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as the secondary antibody at 1:1500 dilution.

      Blocking and diluting buffer: 5% NFDM/TBST.



      All lanes : Anti-TCP1 alpha antibody [EPR4082] (ab109126) at 1/1000 dilution

      Lane 1 : ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate
      Lane 2 : PBS instead of ab109126 in ECV-304 whole cell lysate

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

      Observed band size: 60 kDa (why is the actual band size different from the predicted?)

    • All lanes : Anti-TCP1 alpha antibody [EPR4082] (ab109126) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : ECV-304 cell lysates
      Lane 3 : MCF7 cell lysates
      Lane 4 : Daudi cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 60 kDa

    • ab109126, at 1/100 dilution staining TCP1 in paraffin-embedded Human testis tissue, by Immunohistochemistry.

    文献

    This product has been referenced in:
    • Intawicha P  et al. Proteomic profiling of rabbit embryonic stem cells derived from parthenotes and fertilized embryos. PLoS One 8:e67772 (2013). WB, ICC/IF ; Rabbit . Read more (PubMed: 23861804) »
    • Gao X  et al. Splice isoforms of phosducin-like protein control the expression of heterotrimeric G proteins. J Biol Chem 288:25760-8 (2013). Read more (PubMed: 23888055) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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