RabMAb

Anti-TCP1 alpha抗体[EPR4081] (ab92587)

概述

  • 产品名称Anti-TCP1 alpha抗体[EPR4081]
    参阅全部 TCP1 alpha 一抗
  • 描述
    兔单克隆抗体[EPR4081] to TCP1 alpha
  • 经测试应用适用于: WB, IHC-P, ICC, IPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N terminal of Human TCP1 alpha (UniProt ID: P17987).

  • 阳性对照
    • HeLa, ECV-304, A459 and 293T cell lysates, Human colon tissue, Human kidney tissue and HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab92587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
ICC
IP
  • 应用说明ICC: 1/250 - 1/500.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
    IP: 1/10 - 1/100.
    WB: 1/10000 - 1/50000. Predicted molecular weight: 60 kDa.

    Is unsuitable for Flow Cyt.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
    • 序列相似性Belongs to the TCP-1 chaperonin family.
    • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton > centrosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AI528772 antibody
      • c-cpn antibody
      • CCT alpha antibody
      • CCT antibody
      • CCT-alpha antibody
      • CCT1 antibody
      • Ccta antibody
      • CCTalpha antibody
      • D6S230E antibody
      • MGC133746 antibody
      • p63 antibody
      • T complex 1 antibody
      • T complex protein 1 alpha subunit antibody
      • T complex protein 1 antibody
      • T-complex homolog TCP1 antibody
      • T-complex protein 1 subunit alpha antibody
      • T-complex protein 1 subunit alpha B antibody
      • Tailless complex polypeptide 1 antibody
      • Tailless complex polypeptide 1A antibody
      • Tailless complex polypeptide 1B antibody
      • TCP 1 alpha antibody
      • Tcp-1 antibody
      • TCP-1-alpha antibody
      • TCP1 antibody
      • TCPA_HUMAN antibody
      • Tp63 antibody
      • TRic antibody
      see all

    Anti-TCP1 alpha antibody [EPR4081] 图像

    • All lanes : Anti-TCP1 alpha antibody [EPR4081] (ab92587) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : ECV-304 cell lysate
      Lane 3 : A459 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 60 kDa
    • ab92587 at 1/100 dilution staining TCP1 alpha in (1) Human colon tissue and (2) Human kidney tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
    • ab92587 at 1/250 dilution staining TCP1 alpha in HeLa cells by Immunofluorescence.

    Anti-TCP1 alpha antibody [EPR4081] (ab92587)参考文献

    ab92587 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab92587.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"