The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
应用说明Is unsuitable for WB.
功能Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123508 at 6 µg/mg lysate for IP Subsequent Western blot detection used an antibody which recognized an downstream epitope of TCP1 alpha at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds
Anti-TCP1 alpha antibody (ab123508)参考文献
has not yet been referenced specifically in any publications.