概述

  • 产品名称Anti-TCFL5抗体
    参阅全部 TCFL5 一抗
  • 描述
    山羊多克隆抗体to TCFL5
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide:

    C-KYIQERHGDSLKKE

    , corresponding to internal sequence amino acids 400-413 of Human TCFL5

  • 阳性对照
    • Human Testis lysate

性能

应用

Our Abpromise guarantee covers the use of ab53546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: Peptide ELISA: Antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa). An additional band of unknown identity was also consistently observed at 28kDa. This band was greatly reduced by incubation with the immunising peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Putative transcription factor. Isoform 3 may play a role in early spermatogenesis.
    • 组织特异性Isoform 3 is testis specific. Isoform 2 is pancreas specific.
    • 序列相似性Contains 1 bHLH (basic helix-loop-helix) domain.
    • 发展阶段Isoform 3 is specifically expressed in primary spermatocytes at the pachytene stage, but not those at leptonema stage. Not expressed in other testicular cells, including spermatogonia located in the basal compartment of the seminiferous tubule or spermatids.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • bHLHe82 antibody
      • CHA antibody
      • Cha transcription factor antibody
      • E2BP-1 antibody
      • E2BP1 antibody
      • Figlb antibody
      • HPV-16 E2-binding protein 1 antibody
      • MGC46135 antibody
      • TCFL 5 antibody
      • TCFL5 antibody
      • TCFL5_HUMAN antibody
      • transcription factor-like 5 (basic helix-loop-helix) antibody
      • Transcription factor-like 5 protein antibody
      see all

    Anti-TCFL5 antibody 图像

    • All lanes : ab53546 antibody (0.3µg/ml) staining of TCFL5 in Human Testis lysate (35µg protein in RIPA buffer).

      Lane 1 : Without the immunising peptide
      Lane 2 : With the immunising peptide


      Predicted band size : 48 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

    Anti-TCFL5 antibody (ab53546)参考文献

    ab53546 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"