概述

  • 产品名称
    Anti-TCF7L2抗体[EP2033Y]
    参阅全部 TCF7L2 一抗
  • 描述
    兔单克隆抗体[EP2033Y] to TCF7L2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide. within Human TCF7L2 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q9NQB0

  • 阳性对照
    • Jurkat cell lysate; HeLa cells; human breast carcinoma tissue.
  • 常规说明

     

    Alternative versions available:

    Anti-TCF7L2 antibody (Alexa Fluor® 488) [EP2033Y] (ab206062)
    Anti-TCF7L2 antibody (Alexa Fluor® 647) [EP2033Y] (ab206063)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab76151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/25000 - 1/100000. Predicted molecular weight: 68 kDa.
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/100 - 1/250.

靶标

  • 功能
    Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.
  • 组织特异性
    Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom.
  • 疾病相关
    Note=Constitutive activation and subsequent transactivation of target genes may lead to the maintenance of stem-cell characteristics (cycling and longevity) in cells that should normally undergo terminal differentiation and constitute the primary transforming event in colorectal cancer (CRC).
    Genetic variations in TCF7L2 are associated with susceptibility to non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
  • 序列相似性
    Belongs to the TCF/LEF family.
    Contains 1 HMG box DNA-binding domain.
  • 发展阶段
    Highly expressed in crypt regions and barely detectable in villi in epithelium from fetal small intestine at week 16. At week 22 expression in villi had increased strongly.
  • 结构域
    The promoter-specific activation domain interacts with the transcriptional coactivator EP300.
  • 翻译后修饰
    In vitro, phosphorylated by TNIK.
    Polysumoylated. Sumoylation is enhanced by PIAS family members and desumoylated by AXAM/SENP2. Sumoylation/desumoylation regulates TCF4 transcription activity in the Wnt signaling pathway without altering interaction with CTNNB1 nor binding DNA.
  • 细胞定位
    Nucleus > PML body. Diffuse pattern. Colocalizes with SUMO1 and PIAS4 in a subset of PML (promyelocytic leukemia) nuclear bodies.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • HMG box transcription factor 4 antibody
    • hTCF 4 antibody
    • hTCF-4 antibody
    • T cell factor 4 antibody
    • T cell specific HMG box antibody
    • T cell specific transcription factor 4 antibody
    • T-cell factor 4 antibody
    • T-cell-specific transcription factor 4 antibody
    • TCF 4 antibody
    • TCF-4 antibody
    • TCF4 antibody
    • TCF7L2 antibody
    • TCF7L2 protein antibody
    • TF7L2_HUMAN antibody
    • Transcription factor 7 like 2 antibody
    • Transcription factor 7 like 2 T cell specific HMG box antibody
    • Transcription factor 7-like 2 antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-TCF7L2 antibody [EP2033Y] (ab76151)
    Immunocytochemistry/ Immunofluorescence - Anti-TCF7L2 antibody [EP2033Y] (ab76151)

    Immunofluorescence staining of HT-29 cells with purified ab76151 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

  • Western blot - Anti-TCF7L2 [EP2033Y] antibody (ab76151)
    Western blot - Anti-TCF7L2 [EP2033Y] antibody (ab76151)
    Anti-TCF7L2 antibody [EP2033Y] (ab76151) at 1/2000 dilution + Mouse kidney

    Predicted band size: 68 kDa

  • Western blot - TCF7L2 antibody [EP2033Y] (ab76151)
    Western blot - TCF7L2 antibody [EP2033Y] (ab76151)
    Anti-TCF7L2 antibody [EP2033Y] (ab76151) at 1/100000 dilution + Jurkat cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/1000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCF7L2 antibody [EP2033Y] (ab76151)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCF7L2 antibody [EP2033Y] (ab76151)
    ab76151 at 1/100 dilution staining T cell Transcription Factor 4 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
  • Flow Cytometry - Anti-TCF7L2 antibody [EP2033Y] (ab76151)
    Flow Cytometry - Anti-TCF7L2 antibody [EP2033Y] (ab76151)
    Overlay histogram showing Jurkat cells stained with ab76151 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76151, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

文献

This product has been referenced in:
  • Xiang J  et al. TCF7L2 positively regulates aerobic glycolysis via the EGLN2/HIF-1a axis and indicates prognosis in pancreatic cancer. Cell Death Dis 9:321 (2018). Read more (PubMed: 29476053) »
  • Li FF  et al. Different effection of p.1125Val>Ala and rs11954856 in APC on Wnt signaling pathway. Oncotarget 8:70854-70864 (2017). Read more (PubMed: 29050326) »

See all 8 Publications for this product

发表研究结果有使用 ab76151？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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2 reviews or Q&A

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I was wondering if more specific information was available regarding the specific region of TCF7L2 that antibody ab67151 recognizes. On the website it only indicates that it recognizes near the n terminus.

Thank you for contacting us and your interest in our products. I am pleased to provide the following immunogen information: xxxxxx of human T cell Transcription Factor 4 In order to aid your research we are happy to provide this information ...

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Regarding the TCF7L2 antibody (ab76151)that we ordered and used right now, what is the concentration of this product? I checked the datasheet and online information, but only find that the volume is 100 ul. Since I am doing ChiP using this antibody, I ...

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Thank you for contacting Abcam.

The concentration of the antibody varies slightly from batch to batch, so if you could provide me with the lot number of the vial you have, I would be happy to find out the concentration for you.

I lo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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