Synthetic peptide. within Human TCF7L2 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9NQB0
Alternative versions available:
Anti-TCF7L2 antibody (Alexa Fluor® 488) [EP2033Y] (ab206062)
Anti-TCF7L2 antibody (Alexa Fluor® 647) [EP2033Y] (ab206063)
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab76151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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WB | 1/25000 - 1/100000. Predicted molecular weight: 68 kDa. | |
IP | 1/50. | |
IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
Flow Cyt | 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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ICC/IF | 1/100 - 1/250. |
Immunofluorescence staining of HT-29 cells with purified ab76151 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"