Anti-TCF7L2抗体[0.T.149] (ab32873)

概述

  • 产品名称Anti-TCF7L2抗体[0.T.149]
    参阅全部 TCF7L2 一抗
  • 描述
    小鼠单克隆抗体[0.T.149] to TCF7L2
  • 特异性Recognizes TCF4 at 66kD in Cos1 cells expressing transfected TCF4
  • 经测试应用适用于: ELISA, IHC-P, ICC/IF, IP, WB, EMSAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    His-tagged fusion protein, corresponding to amino acids 31-331 of Human TCF4

  • 阳性对照
    • SW620 whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab32873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 71 kDa).
EMSA Use at an assay dependent concentration.

靶标

  • 功能Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.
  • 组织特异性Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom.
  • 疾病相关Note=Constitutive activation and subsequent transactivation of target genes may lead to the maintenance of stem-cell characteristics (cycling and longevity) in cells that should normally undergo terminal differentiation and constitute the primary transforming event in colorectal cancer (CRC).
    Genetic variations in TCF7L2 are associated with susceptibility to non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
  • 序列相似性Belongs to the TCF/LEF family.
    Contains 1 HMG box DNA-binding domain.
  • 发展阶段Highly expressed in crypt regions and barely detectable in villi in epithelium from fetal small intestine at week 16. At week 22 expression in villi had increased strongly.
  • 结构域The promoter-specific activation domain interacts with the transcriptional coactivator EP300.
  • 翻译后修饰In vitro, phosphorylated by TNIK.
    Polysumoylated. Sumoylation is enhanced by PIAS family members and desumoylated by AXAM/SENP2. Sumoylation/desumoylation regulates TCF4 transcription activity in the Wnt signaling pathway without altering interaction with CTNNB1 nor binding DNA.
  • 细胞定位Nucleus > PML body. Diffuse pattern. Colocalizes with SUMO1 and PIAS4 in a subset of PML (promyelocytic leukemia) nuclear bodies.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HMG box transcription factor 4 antibody
    • hTCF 4 antibody
    • hTCF-4 antibody
    • T cell factor 4 antibody
    • T cell specific HMG box antibody
    • T cell specific transcription factor 4 antibody
    • T-cell factor 4 antibody
    • T-cell-specific transcription factor 4 antibody
    • TCF 4 antibody
    • TCF-4 antibody
    • TCF4 antibody
    • TCF7L2 antibody
    • TCF7L2 protein antibody
    • TF7L2_HUMAN antibody
    • Transcription factor 7 like 2 antibody
    • Transcription factor 7 like 2 T cell specific HMG box antibody
    • Transcription factor 7-like 2 antibody
    see all

Anti-TCF7L2 antibody [0.T.149] 图像

  • All lanes : Anti-TCF7L2 antibody [0.T.149] (ab32873) at 4 µg/ml

    Lane 1 : Cos-1 cells
    Lane 2 : Cos-1 cells transfected with TCF7L2 cDNA in pUSE


    Predicted band size : 71 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

Anti-TCF7L2 antibody [0.T.149] (ab32873)参考文献

This product has been referenced in:
  • Nagasawa M  et al. Development of human plasmacytoid dendritic cells depends on the combined action of the basic helix-loop-helix factor E2-2 and the Ets factor Spi-B. Eur J Immunol 38:2389-400 (2008). Read more (PubMed: 18792017) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - nuclear (Jurkat)
Loading amount 60 µg
Specification Jurkat
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 13 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (T lymphocytes, Jurkat)
Loading amount 60 µg
Specification T lymphocytes, Jurkat
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Xiao Qing Lu

Verified customer

提交于 Apr 03 2009

Thank you for your enquiry. Thank you for highlighting these key differences and confusion in the TCF4 nomenclature to me. I have looked into the details of these antibodies and as you highlight ab2233 was raised against TCF4: a bHLH transcription ...

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