概述

  • 产品名称Anti-TCF4抗体
    参阅全部 TCF4 一抗
  • 描述
    小鼠单克隆抗体to TCF4
  • 经测试应用适用于: Flow Cyt, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Recombinant fragment (GST-tag) corresponding to Human TCF4 aa 1-365.
    Database link: P15884

  • 阳性对照
    • A549 cells

性能

应用

Our Abpromise guarantee covers the use of ab60727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa).
ELISA Use at an assay dependent concentration.

靶标

  • 功能Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.
  • 组织特异性Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain.
  • 疾病相关Defects in TCF4 are a cause of Pitt-Hopkins syndrome (PTHS) [MIM:610954]. PTHS is a rare syndromic encephalopathy characterized by severe psychomotor delay, epilepsy, daily bouts of diurnal hyperventilation starting in infancy, mild postnatal growth retardation, postnatal microcephaly, and distinctive facial features. Since most hitherto reported cases have been sporadic, with males and females equally affected, PTHS is regarded as an autosomal dominant condition.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 结构域the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bHLHb19 antibody
    • Class B basic helix-loop-helix protein 19 antibody
    • E2 2 antibody
    • Immunoglobulin transcription factor 2 antibody
    • ITF 2 antibody
    • ITF-2 antibody
    • ITF2 antibody
    • ITF2_HUMAN antibody
    • MGC149723 antibody
    • MGC149724 antibody
    • PTHS antibody
    • SEF 2 antibody
    • SEF-2 antibody
    • SEF2 1 antibody
    • SEF2 1A antibody
    • SEF2 1B antibody
    • SEF2 antibody
    • SL3 3 enhancer factor 2 antibody
    • SL3-3 enhancer factor 2 antibody
    • TCF 4 antibody
    • TCF-4 antibody
    • TCF4 antibody
    • Transcription factor 4 antibody
    see all

Anti-TCF4 antibody 图像

  • Anti-TCF4 antibody (ab60727) + A549 cells

    Predicted band size : 71 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
  • Overlay histogram showing HeLa cells stained with ab60727 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60727, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-TCF4 antibody (ab60727)参考文献

This product has been referenced in:
  • Kim KH  et al. Wnt/ß-catenin signaling is a key downstream mediator of MET signaling in glioblastoma stem cells. Neuro Oncol 15:161-71 (2013). Human . Read more (PubMed: 23258844) »

See 1 Publication for this product

Product Wall

I can confirm that the product ab60727 is against the TCF4 protein NP_001077431.1 (
http://www.uniprot.org/uniprot/P15884). It seems like the author of the paper have listed the wrong abID. I will be removing the publication from datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"