Anti-TCF3 / E2A抗体(ab69999)
Key features and details
- Rabbit polyclonal to TCF3 / E2A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TCF3 / E2A抗体
参阅全部 TCF3 / E2A 一抗 -
描述
兔多克隆抗体to TCF3 / E2A -
宿主
Rabbit -
特异性
Detects endogenous levels of total TCF3/E2A protein. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Synthesized peptide derived from the C terminus of human TCF3/E2A
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Affinity purified from rabbit antiserum by affinity chromatography, using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab69999于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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IHC-P |
1/50 - 1/100.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
IHC-P
1/50 - 1/100. |
靶标
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功能
Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. -
疾病相关
Note=Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
结构域
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
翻译后修饰
Phosphorylated following NGF stimulation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6929 Human
- Entrez Gene: 171046 Rat
- Omim: 147141 Human
- SwissProt: P15923 Human
- SwissProt: P21677 Rat
- Unigene: 371282 Human
- Unigene: 10290 Rat
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别名
- AGM8 antibody
- bHLHb21 antibody
- Class B basic helix-loop-helix protein 21 antibody
see all
图片
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All lanes : Anti-TCF3 / E2A antibody (ab69999) at 1/500 dilution
Lane 1 : Extracts from 293 cells, minus immunising peptide
Lane 2 : Extracts from 293 cells, plus immunising peptide 5µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 68 kDa
Observed band size: 68 kDa -
ab69999, at a 1/50 dilution, staining human TCF3/E2A in brain, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue, minus immunising peptide (left image) and plus immunising peptide (right image).
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab69999 被引用在 5 文献中.
- Song Y et al. Interleukin-6 (IL-6) Activates the NOTCH1 Signaling Pathway Through E-Proteins in Endometriotic Lesions. J Clin Endocrinol Metab 105:N/A (2020). PubMed: 32119078
- Ahmed NS et al. Epidermal E-Cadherin Dependent ß-Catenin Pathway Is Phytochemical Inducible and Accelerates Anagen Hair Cycling. Mol Ther 25:2502-2512 (2017). PubMed: 28803863
- Loriot C et al. Deciphering the molecular basis of invasiveness in Sdhb-deficient cells. Oncotarget 6:32955-65 (2015). WB . PubMed: 26460615
- Li C et al. Hypomethylation-associated up-regulation of TCF3 expression and recurrence in stage II and III colorectal cancer. PLoS One 9:e112005 (2014). IHC ; Human . PubMed: 25375219
- Lee K et al. Snail1, Snail2, and E47 promote mammary epithelial branching morphogenesis. EMBO J 30:2662-74 (2011). IF, WB ; Mouse . PubMed: 21610693