概述

  • 产品名称Anti-TCF21抗体- ChIP Grade
    参阅全部 TCF21 一抗
  • 描述
    兔多克隆抗体to TCF21 - ChIP Grade
  • 特异性ab32981 recognises Transcription factor 21 (TCF21).
  • 经测试应用适用于: IHC-P, ChIP, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide derived from a region between amino acids 42-91 of Human TCF21. Note: The exact sequence is proprietary.

  • 阳性对照

性能

应用

Our Abpromise guarantee covers the use of ab32981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 231121635µg used per ChIP
ELISA 1/12500.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 20 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-TCF21 antibody - ChIP Grade 图像

  • Anti-TCF21 antibody - ChIP Grade (ab32981) at 0.5 µg/ml + fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 20 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry of Human Brain, cortex, senile plaque tissue at an antibody concentration of 5.0µg/ml using anti-TCF21 antibody (ab32981)

Anti-TCF21 antibody - ChIP Grade (ab32981)参考文献

This product has been referenced in:
  • Risebro CA  et al. Characterisation of the human embryonic and foetal epicardium during heart development. Development 142:3630-6 (2015). IHC ; Human . Read more (PubMed: 26395486) »
  • Harel I  et al. Pharyngeal mesoderm regulatory network controls cardiac and head muscle morphogenesis. Proc Natl Acad Sci U S A 109:18839-44 (2012). ChIP ; Mouse . Read more (PubMed: 23112163) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (squamous cell carcinoma, tongue)
Specification squamous cell carcinoma, tongue
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
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提交于 Jul 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"