概述

性能

应用

Our Abpromise guarantee covers the use of ab32981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 23112163

   5 µg used per ChIP

ELISA 1/12500.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 20 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Immunohistochemistry of Human Brain, cortex, senile plaque tissue at an antibody concentration of 5.0µg/ml using anti-TCF21 antibody (ab32981)

  • Anti-TCF21 antibody - ChIP Grade (ab32981) at 0.5 µg/ml + fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size: 20 kDa
    Observed band size: 17 kDa (why is the actual band size different from the predicted?)

文献

This product has been referenced in:
  • Gooskens SL  et al. TCF21 hypermethylation regulates renal tumor cell clonogenic proliferation and migration. Mol Oncol 12:166-179 (2018). WB ; Human . Read more (PubMed: 29080283) »
  • Suffee N  et al. Atrial natriuretic peptide regulates adipose tissue accumulation in adult atria. Proc Natl Acad Sci U S A 114:E771-E780 (2017). Read more (PubMed: 28096344) »

See all 6 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (squamous cell carcinoma, tongue)
Specification
squamous cell carcinoma, tongue
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

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Verified customer

提交于 Jul 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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